SPN siRNA (Mouse)
Référence MBS827961-15nmol
Conditionnement : 15nmol
Marque : MyBiosource
SPN siRNA – Leukosialin (Mouse)
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS827961MBS827961 is an siRNA targeting SPN. Also known as SPN siRNA (Mouse). Reactive in Mouse. Host: Synthetic. siRNA to inhibit SPN expression using RNA interference More details
Product Overview
Online Datasheet in PDFProduct Name
SPN, siRNA
Full Product Name
SPN siRNA (Mouse)
Product Gene Name
SPN sirna
Product Synonym Names
Leukosialin; B-cell differentiation antigen LP-3; Leukocyte sialoglycoprotein; Lymphocyte antigen 48; Ly-48; Sialophorin; CD antigen CD43
Host
Synthetic
Species Reactivity
Mouse
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
SPN siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
40,038 Da
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SPN gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for SPN sirna
RNA Interference (RNAi)
Other Notes
Small volumes of SPN sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for SPN. It may not necessarily be applicable to this product.
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
leukosialin
NCBI Official Synonym Full Names
sialophorin
NCBI Official Symbol
Spn
NCBI Official Synonym Symbols
Cd43; Ly48; Galgp; Ly-48; A630014B01Rik
UniProt Protein Name
Leukosialin
UniProt Synonym Protein Names
B-cell differentiation antigen LP-3; Leukocyte sialoglycoprotein; Lymphocyte antigen 48; Ly-48; Sialophorin; CD_antigen: CD43
Protein Family
Leukosialin
UniProt Gene Name
Spn
UniProt Synonym Gene Names
Ly-48
UniProt Entry Name
LEUK_MOUSE
NCBI Protein Information
leukosialin
UniProt Comments for SPN
sialophorin: One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter-receptor for SN/Siglec-1. During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response. Interacts with HIPK2 via the cytoplasmic domain. Interacts with RDX. Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.
Protein type: Membrane protein, integral; Cell surface
Cellular Component: extracellular space; microvillus; cell surface; membrane; integral to plasma membrane; integral to membrane; basement membrane; uropod; external side of plasma membrane; cleavage furrow
Molecular Function: protein domain specific binding; protein binding
Biological Process: positive regulation of tumor necrosis factor biosynthetic process; regulation of immune response; regulation of defense response to virus; negative regulation of cell adhesion; negative thymic T cell selection; cell surface receptor linked signal transduction; response to protozoan; negative regulation of T cell proliferation; T cell costimulation; defense response to bacterium; positive regulation of T cell proliferation; positive regulation of protein amino acid phosphorylation; negative regulation of type IV hypersensitivity; negative regulation of T cell activation
Show more Protein type: Membrane protein, integral; Cell surface
Cellular Component: extracellular space; microvillus; cell surface; membrane; integral to plasma membrane; integral to membrane; basement membrane; uropod; external side of plasma membrane; cleavage furrow
Molecular Function: protein domain specific binding; protein binding
Biological Process: positive regulation of tumor necrosis factor biosynthetic process; regulation of immune response; regulation of defense response to virus; negative regulation of cell adhesion; negative thymic T cell selection; cell surface receptor linked signal transduction; response to protozoan; negative regulation of T cell proliferation; T cell costimulation; defense response to bacterium; positive regulation of T cell proliferation; positive regulation of protein amino acid phosphorylation; negative regulation of type IV hypersensitivity; negative regulation of T cell activation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with SPN sirna
| Reference Product | PubMed Publications |
|---|---|
| Cd44 sirna | >7 publications with SPN and Cd44 |
| Siglec1 sirna | >1 publications with SPN and Siglec1 |
Pathways associated with SPN sirna
| Products by Pathway | Pathway Diagram |
|---|---|
| Basigin Interactions Pathway antibodies | Basigin Interactions Pathway Diagram |
| Cell Adhesion Molecules (CAMs) Pathway antibodies | Cell Adhesion Molecules (CAMs) Pathway Diagram |
| Cell Adhesion Molecules (CAMs) Pathway antibodies | Cell Adhesion Molecules (CAMs) Pathway Diagram |
| Cell Surface Interactions At The Vascular Wall Pathway antibodies | Cell Surface Interactions At The Vascular Wall Pathway Diagram |
| Epstein-Barr Virus Infection Pathway antibodies | Epstein-Barr Virus Infection Pathway Diagram |
| Epstein-Barr Virus Infection Pathway antibodies | Epstein-Barr Virus Infection Pathway Diagram |
| Hemostasis Pathway antibodies | Hemostasis Pathway Diagram |
| XPodNet - Protein-protein Interactions In The Podocyte Expanded By STRING Pathway antibodies | XPodNet - Protein-protein Interactions In The Podocyte Expanded By STRING Pathway Diagram |
Diseases associated with SPN sirna
| Disease Name | Pubmed Publications |
|---|---|
| Lung Diseases Antibodies | >234 publications with SPN and Lung Diseases |
| Nervous System Diseases Antibodies | >90 publications with SPN and Nervous System Diseases |
| Inflammation Antibodies | >46 publications with SPN and Inflammation |
| Cardiovascular Diseases Antibodies | >29 publications with SPN and Cardiovascular Diseases |
| Necrosis Antibodies | >27 publications with SPN and Necrosis |
| Pneumonia Antibodies | >27 publications with SPN and Pneumonia |
| Disease Models, Animal Antibodies | >27 publications with SPN and Disease Models, Animal |
| Hypertension Antibodies | >20 publications with SPN and Hypertension |
| Liver Neoplasms Antibodies | >18 publications with SPN and Liver Neoplasms |
| Breast Neoplasms Antibodies | >10 publications with SPN and Breast Neoplasms |
Organs/Tissues associated with SPN sirna
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Blood Antibodies | >285 publications with SPN and Blood |
| Bone Antibodies | >83 publications with SPN and Bone |
| Bone Marrow Antibodies | >52 publications with SPN and Bone Marrow |
| Liver Antibodies | >50 publications with SPN and Liver |
| Spleen Antibodies | >50 publications with SPN and Spleen |
| Thymus Antibodies | >25 publications with SPN and Thymus |
| Inner Ear Antibodies | >6 publications with SPN and Inner Ear |
| Embryonic Tissue Antibodies | >5 publications with SPN and Embryonic Tissue |
| Adipose Tissue Antibodies | >1 publications with SPN and Adipose Tissue |
| Uncharacterized Tissue Antibodies | >1 publications with SPN and Uncharacterized Tissue |