Succinate Dehydrogenase (SDH) Activity Assay Kit (Colorimetric)
Référence MBS9719205-96
Conditionnement : 96Tests
Marque : MyBiosource
Succinate Dehydrogenase (SDH) Activity (SDH) Assay Kit
For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS9719205PROMOTIONAL PRICING: 5% OFF web . Discount is not applicable for bulk pricing such as 5x96T or 10x96T. Discount will be applied after order has been placed
Principle of the Assay: Succinate Dehydrogenase (SDH) is widely present in animals, plants, microorganisms and cultured cells. SDH is a marker enzyme of mitochondria and a membrane-bound enzyme located on the inner membrane of mitochondria. Also, it is one of… More details
Product Overview
Online Datasheet in PDFProduct Name
Succinate Dehydrogenase (SDH) Activity, Assay Kit
Full Product Name
Succinate Dehydrogenase (SDH) Activity Assay Kit (Colorimetric)
Product Gene Name
SDH assay kit
Samples
Animal and Plant Tissues, Cells and Bacteria
Preparation and Storage
The kit is valid for 6 months. Please refer to the table below Materials supplied and Storage conditions to store all the components.
Shipping||Gel pack with blue ice.
Shipping||Gel pack with blue ice.
Usage Notes
deltaA of Test Tube should be between 0.01-0.3, the sample should be diluted further if deltaA of Test Tube >0.3.
Do not mix the components between different batch numbers and manufacturers; otherwise, the results may be abnormal.
Avoid bubbles while mixing or redissolving components.
Change pipette tips frequently to avoid cross contamination between components.
Ensure that all components and equipment are at the proper temperature before starting the experiment.
All samples and reagents should be on ice to avoid denaturation and deactivation.
Don't recommend to test too many samples in one time,because enzyme activity is calculated by the variation of the absorbance value per unit time.
Recommended to calculate the enzyme activity by protein concentration. If you need to calculate by the sample weight, the total enzyme activity is equal to the enzyme activity of the supernatant and pellet which is cytoplasmic extraction.
Please to extract SDH from fresh samples in order to ensure the enzyme activity.
it is normal while there is fewer precipitates in Reagent III solution. Please filter if the precipitates interfer the result.
Please reduce the tissues weight if the OD values is too high. Or reduce the volume of Extraction Buffer if the OD values is too low.
Do not mix the components between different batch numbers and manufacturers; otherwise, the results may be abnormal.
Avoid bubbles while mixing or redissolving components.
Change pipette tips frequently to avoid cross contamination between components.
Ensure that all components and equipment are at the proper temperature before starting the experiment.
All samples and reagents should be on ice to avoid denaturation and deactivation.
Don't recommend to test too many samples in one time,because enzyme activity is calculated by the variation of the absorbance value per unit time.
Recommended to calculate the enzyme activity by protein concentration. If you need to calculate by the sample weight, the total enzyme activity is equal to the enzyme activity of the supernatant and pellet which is cytoplasmic extraction.
Please to extract SDH from fresh samples in order to ensure the enzyme activity.
it is normal while there is fewer precipitates in Reagent III solution. Please filter if the precipitates interfer the result.
Please reduce the tissues weight if the OD values is too high. Or reduce the volume of Extraction Buffer if the OD values is too low.
Product Categories/Family for SDH assay kit
Biochemical Assay Kit; Cell Metabolism; Cytology
Other Notes
Small volumes of SDH assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for SDH purchase
MBS9719205 is an assay kit targeting SDH. Also known as Succinate Dehydrogenase (SDH) Activity Assay Kit (Colorimetric).
Additional Details
NCBI/Uniprot data below describe general gene information for SDH. It may not necessarily be applicable to this product.
Protein Family
Probable saccharopine dehydrogenase
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.