T4 Polynucleotide Kinase

This product is a recombinant protein obtained by constructing phage T4 Polynucleotide Kinase (T4 PNK) gene into a plasmid and purifying it by expression in E.coli. T4 PNK is a polyribonucleotide 5’-hydroxykinase that catalyzes the transfer of the γ-phosphate group of ATP to the 5’-hydroxy terminus of oligonucleotide chains (double- or single-stranded DNA or RNA). T4 PNK catalyzes the exchange of 5’-P-oligo/polynucleotides for the terminal 5’-phosphate group of ATP when both ATP and ADP are present in appropriate amounts. This product has 3’-end phosphatase activity and can catalyze the hydrolysis of the 3’-phosphate groups of deoxyribonucleoside 3’-monophosphate, deoxyribonucleoside 3’,5’-diphosphate and 3’-phosphate polynucleotides. groups.