B2016434 | T4 Polynucleotide Kinase (PNK) Clinisciences

T4 Polynucleotide Kinase (PNK)

Catalog Number: B2016434 (250 U)
T4 Polynucleotide Kinase (PNK) is a high quality kinase which exhibits 5’ polynucleotide kinase and 3’ phosphatase activity which catalyzes 5’-phosphorylation of DNA and oligonucleotides via transferring the terminal phosphate of ATP to a 5’ hydroxyl group of a nucleic acid. This product has been used as a molecular tool for various biochemical applications. It has also been used in a wide array of other chemical and immunological applications. Custom bulk amounts of this product are available upon request.

Live enquiry about this product via Text/SMS: 1-858-900-3210.

SKU: B2016434 Categories: Peptides, Proteins Tag: MD 1000

Product Description

5/5 - (2 votes)

T4 Polynucleotide Kinase (PNK)
Catalog number: B2016434
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 250 U
Molecular Weight or Concentration: 34.6 kDa
Supplied as: Solution
Applications: a molecular tool for various biochemical applications
Storage: -20 °C
Keywords: T4 Polynucleotide Kinase (PNK)
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Gao M, Guo J, Song Y, Zhu Z, Yang CJ. Detection of T4 Polynucleotide Kinase via Allosteric Aptamer Probe Platform ACS Appl Mater Interfaces. 2017 Nov 8;9(44):38356-38363.
2: Tao M, Shi Z, Cheng R, Zhang J, Li B, Jin Y. Highly specific fluorescence detection of T4 polynucleotide kinase activity via photo-induced electron transfer Anal Biochem. 2015 Sep 15;485:18-24.
3: Durand S, Richard G, Bontems F, Uzan M. Bacteriophage T4 polynucleotide kinase triggers degradation of mRNAs Proc Natl Acad Sci U S A. 2012 May 1;109(18):7073-8.
4: Tao J, Liu Z, Zhu Z, Zhang Y, Wang H, Pang P, Yang C, Yang W. Electrochemical detection of T4 polynucleotide kinase activity based on magnetic Fe(3)O(4)@TiO(2) nanoparticles triggered by a rolling circle amplification strategy Talanta. 2022 May 1;241:123272.
5: Lian S, Liu C, Zhang X, Wang H, Li Z. Detection of T4 polynucleotide kinase activity based on cationic conjugated polymer-mediated fluorescence resonance energy transfer Biosens Bioelectron. 2015 Apr 15;66:316-20.
6: Wu Y, Yi J, Su A, Zhang Y, Wang H, Yang L, Yang W, Pang P. An electrochemical biosensor for T4 polynucleotide kinase activity identification according to host-guest recognition among phosphate pillar[5]arene@palladium nanoparticles@reduced graphene oxide nanocomposite and toluidine blue Mikrochim Acta. 2023 Sep 16;190(10):394.
7: Wei L, Kong X, Wang M, Zhang Y, Pan R, Cheng Y, Lv Z, Zhou J, Ming J. A label-free T4 polynucleotide kinase fluorescence sensor based on split dimeric G-quadruplex and ligation-induced dimeric G-quadruplex/thioflavin T conformation Anal Bioanal Chem. 2022 Nov;414(27):7923-7933.
8: Sun J, Guo A, Yan M, Wu X, Wang GL. Kanamycin triggered nanozyme for homogeneous and amplified colorimetric detection of T4 polynucleotide kinase Talanta. 2023 May 15;257:124335.
9: Shang J, Yu S, Chen Y, Gao Y, Hong C, Li F, Wang F. Real-Time Investigation of Intracellular Polynucleotide Kinase Using a Cascaded Amplification Circuit Anal Chem. 2021 Nov 23;93(46):15559-15566.
10: Zhang Y, Cai Q, Yan X, Jie G. Versatile fluorescence detection of T4 PNK and mRNA based on unique DNA nanomachine amplification Anal Chim Acta. 2023 Apr 22;1251:341003.

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