Total Cholesterol and Cholesteryl Ester Fluorometric Assay Kit

Intended Uses: This kit can be used for determination of Total Cholesterol (TC) and Cholesterol Esters (CE) content in serum, plasma, animal tissue and cell samples.

Principle of the Assay: Total Cholesterol (TC) includes free cholesterol (FC) and cholesteryl esters (CE). Cholesterol ester can be hydrolyzed by cholesterol esterase to produce cholesterol and free fatty acid. Cholesterol is oxidized by cholesterol oxidase to produce delta4-cholestenone and hydrogen peroxide. In the presence of the enzyme and probe, hydrogen peroxide can be catalyzed to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 590 nm is proportional to the cholesterol concentration.

Background/Introduction: Cholesterol is a kind of sterol and lipid in cell membrane. Most of cholesterol in blood exists in the form of cholesterol ester. Lecithin-cholesterol acyltransferase in human plasma is an enzyme that catalyzes the formation of cholesterol ester. Cholesterol synthesized or deposited in peripheral cells returns to the liver through the reverse cholesterol transport system for reusing or regaining bile acids.

Small volumes of Total Cholesterol and Cholesteryl Ester assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2567666 is an assay kit targeting Total Cholesterol and Cholesteryl Ester. Also known as Total Cholesterol and Cholesteryl Ester Fluorometric Assay Kit.