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> Tris Buffer Saline with Tween, 10X (TBST)

Tris Buffer Saline with Tween, 10X (TBST)

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Référence orb2652894

Conditionnement : 1000ml

Marque : Biorbyt

Tris Buffer Saline with Tween, 10X (TBST)

Tris Buffer Saline with Tween, 10X (TBST)

SKU: orb2652894

Description

This buffer is supplied as 10X solution. It is used as 1X in all western blotting, ELISA and Chemiluminescence assays as a washing buffer, making blocking solution, diluting HRP-secondary and primary antibody etc.

Research Area

Signal Transduction

Images & Validation

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Tris Buffer Saline with Tween, 10X (TBST)
Application Notes
Preparation: Dilute this 10X buffer 10 times (e.g. 90 ml of deionized or distilled water + 10 ml of this buffer), mix well, and store 1 X buffer solution at room temperature or 2-8°C for several weeks. Application: Wash membrane or ELISA plates for 10 minutes each for 3 times. For 10x10 cm membrane use at least 50 ml buffer with shaking gently. This buffer is used in between steps in immunoassays protocol. For diluting blocking solution, primary antibody or HRP-secondary antibody, please refer to WB or ELISA protocol.

Key Properties

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Storage & Handling

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StorageRoom Temperature
Concentration10 X
Expiration Date12 months from date of receipt.
DisclaimerFor research use only
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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol