Ultra Sensitive Assay for Nitric Oxide Synthase
Référence MBS480447-1
Conditionnement : 1Kit
Marque : MyBiosource
Nitric Oxide Synthase (NOS3) Assay Kit
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS480447Principle of the Assay: NADPH and L-arginine are required for the continual operation of NOS and production of nitric oxide (NO). In aqueous solution, NO rapidly degrades to nitrate and nitrite. Spectrophotometric quantization of nitrite using Griess Reagent… More details
Product Overview
Product Name
Nitric Oxide Synthase (NOS3), Assay Kit
Full Product Name
Ultra Sensitive Assay for Nitric Oxide Synthase
Product Gene Name
NOS3 assay kit
Product Synonym Names
Ultrasensitive Colorimetric NOS Assay
Preparation and Storage
1. Store this kit and its components according to the label.
- The Nitrate Reductase, NADPH Part A and NADPH Part B should be stored at At -20 degree C.
- - The reconstituted Nitrate Reductase may be stored at At -20 degree C for up to six months.
- - The diluted standard curve may be stored at 4 degree C for up to 24 hours.
- - All other kit components should be stored at 4 degree C.
- The Nitrate Reductase, NADPH Part A and NADPH Part B should be stored at At -20 degree C.
- - The reconstituted Nitrate Reductase may be stored at At -20 degree C for up to six months.
- - The diluted standard curve may be stored at 4 degree C for up to 24 hours.
- - All other kit components should be stored at 4 degree C.
Product References and Citations for NOS3 assay kit
1. Ghigo, D., et al. (2006). Nitric Oxide 15:148-153
2. Schmidt, H.H., et al. (1995). Biochemica 2:22-23
2. Schmidt, H.H., et al. (1995). Biochemica 2:22-23
Other Notes
Small volumes of NOS3 assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for NOS3 purchase
MBS480447 is an assay kit targeting NOS3. Also known as Ultra Sensitive Assay for Nitric Oxide Synthase.
Additional Details
NCBI/Uniprot data below describe general gene information for NOS3. It may not necessarily be applicable to this product.
NCBI Official Full Name
nitric oxide synthase, partial
NCBI Official Synonym Full Names
nitric oxide synthase 3 (endothelial cell)
NCBI Official Symbol
NOS3
NCBI Official Synonym Symbols
eNOS; ECNOS
UniProt Protein Name
Nitric oxide synthase, endothelial
UniProt Synonym Protein Names
Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII
Protein Family
Nitric oxide synthase
UniProt Gene Name
NOS3
UniProt Synonym Gene Names
cNOS; eNOS; NOSIII
UniProt Entry Name
NOS3_HUMAN
NCBI Protein Information
nitric oxide synthase, endothelial; EC-NOS; NOS type III; NOSIII; cNOS; constitutive NOS; endothelial NOS; nitric oxide synthase 3 transcript variant eNOS-delta20; nitric oxide synthase 3 transcript variant eNOS-delta20-21; nitric oxide synthase 3 transcript variant eNOS-delta21
NCBI Summary for NOS3
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]
Show more UniProt Comments for NOS3
eNOS: endothelial constitutive nitric oxide synthase. Synthesizes nitric oxide (NO) from arginine and oxygen, which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Induced in humans by caloric restriction. Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN.
Protein type: Oxidoreductase; Amino Acid Metabolism - arginine and proline; EC 1.14.13.39
Chromosomal Location of Human Ortholog: 7q36
Cellular Component: Golgi membrane; cytoskeleton; cytoplasm; plasma membrane; caveola; nucleus; cytosol
Molecular Function: calmodulin binding; actin monomer binding; protein binding; FAD binding; nitric-oxide synthase activity; NADPH-hemoprotein reductase activity; FMN binding; iron ion binding; heme binding; NADP binding; cadmium ion binding
Biological Process: regulation of systemic arterial blood pressure by endothelin; negative regulation of potassium ion transport; negative regulation of muscle hyperplasia; positive regulation of vasodilation; nitric oxide metabolic process; negative regulation of cell proliferation; regulation of the force of heart contraction by chemical signal; negative regulation of hydrolase activity; regulation of blood pressure; lipopolysaccharide-mediated signaling pathway; negative regulation of blood pressure; angiogenesis; regulation of blood vessel size; nitric oxide biosynthetic process; interaction with host; mitochondrion organization and biogenesis; negative regulation of calcium ion transport; in utero embryonic development; positive regulation of guanylate cyclase activity; regulation of sodium ion transport; ovulation from ovarian follicle; positive regulation of angiogenesis; response to heat; arginine catabolic process; endothelial cell migration; blood vessel remodeling; regulation of nitric-oxide synthase activity; blood coagulation; vascular endothelial growth factor receptor signaling pathway; nitric oxide mediated signal transduction; lung development; smooth muscle hyperplasia
Disease: Preeclampsia/eclampsia 1; Alzheimer Disease; Hypertension, Essential; Stroke, Ischemic
Show more Protein type: Oxidoreductase; Amino Acid Metabolism - arginine and proline; EC 1.14.13.39
Chromosomal Location of Human Ortholog: 7q36
Cellular Component: Golgi membrane; cytoskeleton; cytoplasm; plasma membrane; caveola; nucleus; cytosol
Molecular Function: calmodulin binding; actin monomer binding; protein binding; FAD binding; nitric-oxide synthase activity; NADPH-hemoprotein reductase activity; FMN binding; iron ion binding; heme binding; NADP binding; cadmium ion binding
Biological Process: regulation of systemic arterial blood pressure by endothelin; negative regulation of potassium ion transport; negative regulation of muscle hyperplasia; positive regulation of vasodilation; nitric oxide metabolic process; negative regulation of cell proliferation; regulation of the force of heart contraction by chemical signal; negative regulation of hydrolase activity; regulation of blood pressure; lipopolysaccharide-mediated signaling pathway; negative regulation of blood pressure; angiogenesis; regulation of blood vessel size; nitric oxide biosynthetic process; interaction with host; mitochondrion organization and biogenesis; negative regulation of calcium ion transport; in utero embryonic development; positive regulation of guanylate cyclase activity; regulation of sodium ion transport; ovulation from ovarian follicle; positive regulation of angiogenesis; response to heat; arginine catabolic process; endothelial cell migration; blood vessel remodeling; regulation of nitric-oxide synthase activity; blood coagulation; vascular endothelial growth factor receptor signaling pathway; nitric oxide mediated signal transduction; lung development; smooth muscle hyperplasia
Disease: Preeclampsia/eclampsia 1; Alzheimer Disease; Hypertension, Essential; Stroke, Ischemic
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Pathways associated with NOS3 assay kit
| Products by Pathway | Pathway Diagram |
|---|---|
| ACE Inhibitor Pathway antibodies | ACE Inhibitor Pathway Diagram |
| AGE/RAGE Pathway antibodies | AGE/RAGE Pathway Diagram |
| Angiogenesis Pathway antibodies | Angiogenesis Pathway Diagram |
| Angiopoietin Receptor Tie2-mediated Signaling Pathway antibodies | Angiopoietin Receptor Tie2-mediated Signaling Pathway Diagram |
| Arginine And Proline Metabolism Pathway antibodies | Arginine And Proline Metabolism Pathway Diagram |
| Arginine And Proline Metabolism Pathway antibodies | Arginine And Proline Metabolism Pathway Diagram |
| Calcium Signaling Pathway antibodies | Calcium Signaling Pathway Diagram |
| Calcium Signaling Pathway antibodies | Calcium Signaling Pathway Diagram |
| Corticotropin-releasing Hormone Pathway antibodies | Corticotropin-releasing Hormone Pathway Diagram |
| Disease Pathway antibodies | Disease Pathway Diagram |
Diseases associated with NOS3 assay kit
| Disease Name | Pubmed Publications |
|---|---|
| Cardiovascular Diseases Antibodies | >2226 publications with NOS3 and Cardiovascular Diseases |
| Hypertension Antibodies | >1092 publications with NOS3 and Hypertension |
| Myocardial Ischemia Antibodies | >590 publications with NOS3 and Myocardial Ischemia |
| Ischemia Antibodies | >582 publications with NOS3 and Ischemia |
| Atherosclerosis Antibodies | >476 publications with NOS3 and Atherosclerosis |
| Reperfusion Injury Antibodies | >295 publications with NOS3 and Reperfusion Injury |
| Myocardial Infarction Antibodies | >264 publications with NOS3 and Myocardial Infarction |
| Coronary Artery Disease Antibodies | >261 publications with NOS3 and Coronary Artery Disease |
| Cerebral Infarction Antibodies | >215 publications with NOS3 and Cerebral Infarction |
| Diabetes Mellitus, Experimental Antibodies | >209 publications with NOS3 and Diabetes Mellitus, Experimental |
Organs/Tissues associated with NOS3 assay kit
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Vascular Antibodies | >3734 publications with NOS3 and Vascular |
| Blood Antibodies | >2838 publications with NOS3 and Blood |
| Heart Antibodies | >1297 publications with NOS3 and Heart |
| Kidney Antibodies | >554 publications with NOS3 and Kidney |
| Brain Antibodies | >458 publications with NOS3 and Brain |
| Lung Antibodies | >404 publications with NOS3 and Lung |
| Liver Antibodies | >281 publications with NOS3 and Liver |
| Embryonic Tissue Antibodies | >214 publications with NOS3 and Embryonic Tissue |
| Bone Antibodies | >158 publications with NOS3 and Bone |
| Skin Antibodies | >103 publications with NOS3 and Skin |
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