Williams' Medium E w/o L-Glutamine, w/o Amino Acids (Powder)

Light orange, homogeneous, free flowing powder

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Yellow, clear, complete

4.9

<1EU/ml

Dissolve 9.73 grams per liter of distilled/deionized (DDI) water. Heat with stirring until completely solubilized. Adjust pH as necessary. If required, add sodium bicarbonate with stirring. Sterilize with 0.2um membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Inorganic Salts:
Calcium Chloride•2H2O 0.265
Cupric Sulfate•H2O 0.0000001
Ferric Nitrate•9H2O 0.0000001
Magnesium Sulfate 0.0977
Manganese Chloride•4H2O 0.0000001
Potassium Chloride 0.4
Sodium Chloride 6.8
Sodium Phosphate Monobasic 0.122
Zinc Sulfate•7H2O 0.0000002

Amino Acids:
L-Alanine Absent
L-Arginine Absent
L-Aspartic Acid Absent
L-Asparagine•H2O Absent
L-Cysteine (free acid) Absent
L-Cystine•2HCl Absent
L-Glutamic Acid Absent
L-Glutamine Absent
Glycine Absent
L-Histidine Absent

Amino Acids:
L-Isoleucine Absent
L-Leucine Absent
L-Lysine•HCl Absent
L-Methionine Absent
L-Phenylalanine Absent
L-Proline Absent
L-Serine Absent
L-Threonine Absent
L-Tryptophan Absent

L-Tyrosine•2Na•2H2O Absent
L-Valine Absent

Vitamins:
Ascorbic Acid•Na 0.00227
D-Biotin 0.0005
Choline Chloride 0.0015
Vitamin B-12 0.0002
Calciferol 0.0001
Folic Acid 0.001
myo-Inositol 0.002
Menadione (sodium bisulfate) 0.00001
Niacinamide 0.001
D-Pantothenic Acid•Ca 0.001
Pyridoxal•HCl 0.001
Riboflavin 0.0001
Thiamine•HCl 0.001
DL-a-Tocopherol Phosphate•2Na 0.00001
Retinol Acetate 0.0001

Other:
D-Glucose 2.00
Glutathione 0.00005
Methyl Linoleate 0.00003
Phenol Red, Sodium 0.0107
Pyruvic Acid, Sodium 0.025

Total: 9.73g/L

Culture Media; Media-Cell Culture; Williams'