NB-21-23859 | XtenCHO Race Expression medium Clinisciences

Note:: For research use only.

XtenCHO Race outperforms up to 6X (Cixutumumab and Peresolimab)

Specifications XtenCHO ™ Expression medium

Product name	XtenCHO ™ Expression medium
Expression system	Mammalian
Delivery condition	Blue ice (+4°C)
Delivery lead time in business days	Europe: 2-4 business days USA & Canada: 2-4 business days Rest of the world: 5-10 business days
Storage condition	4°C
Brand	ProteoGenix
Host species	Mammalian cells
Reference	PX-XTE-002
Note	For research use only.
Kit Content	1L bottle
Limited Use Label Licenses	Limited Use Label License for internal Research and Development Use of Mammalian Cell Lines

Description

XtenCHO™ Expression Medium

The XtenCHO™ Expression Medium is specifically designed and optimized for small to medium-scale recombinant protein production in Chinese Hamster Ovary cells (CHO). This medium is serum free, animal component free and chemically-defined. This chemical definition enables a high batch to batch reproducibility and reliability. Moreover, the serum free medium allows very high transfection efficiency. This medium optimizes productivity and sustains balanced growth and viability of transfected cells in suspension. In combination with the XtenCHO™ Race Cells and the XtenCHO™ Race transfection Kit , it has achieved to reach a production yield of 904mg/L for recombinant antibody production! This makes this system the leader of its market.

XtenCHO™ Expression Medium use

This medium has proven highly efficient for transient transfection of XtenCHO™ Race Cells from a few mL to 10L production volume. It can be used for the transfection as well as for the sub culturing of cells, making experiment easier for researchers. The protocols provided with the XtenCHO™ Race Starter Kit shows how to use the XtenCHO™ Expression Medium in a simple and adaptable manner to better suit your lab and needs. For transfection protocol XtenCHO™ Expression Medium requires additional supplementation of L-Glutamine (8mM) and Anti-clumping agent (0.5%). L-Glutamine and Anti-clumping agent are supplementary key elements to reach maximal production yield. However L-Glutamine is quickly hydrolyzed in medium, decreasing its actual concentration and generating byproducts of hydrolysis which can be toxic to the cells. The Anti-clumping agent should not be added in the XtenCHO™ Expression Medium during the transfection or it would result in decreased protein yields. It is recommended to use XtenCHO™ Expression Medium for cultures in disposable, sterile, vented, baffled Erlenmeyer shake flask with incubator shaker set at 37°C with ≥80% relative humidity and 5% CO2.
The XtenCHO™ Expression Medium is a major component of the XtenCHO™ Race Starter Kit. It can also be delivered worldwide separately at your convenience.

Documents

QC & Validation

XtenCHO Race outperforms up to 6X (Cixutumumab and Peresolimab)

Crude production yields in mg/L obtained in 24-well plates cultures without feeding (Octet Analysis) Yields in flask (better aeration) will be 1.5 to 2.5 Higher for Human/humanized IgG1/IgG4 (depending on the antibody considered)

3D Representation

Publications

Luo K, Chen Y, Wang F. Shrimp Plasma MANF Works as an Invertebrate Anti-Inflammatory Factor via a Conserved Receptor Tyrosine Phosphatase. J Immunol. 2022 Mar 1;208(5):1214-1223. doi: https://doi.org/10.4049/jimmunol.2100595. Epub 2022 Feb 11. PMID: 35149533.
Bonduelle O, Chaudesaigues C, Tolazzi M, Suleiman E, de Bernard S, Alves K, Nourikyan J, Bohec M, Baudrin LG, Katinger D, Debré P, Scarlatti G, Vieillard V, Combadière B. Dichotomy in Neutralizing Antibody Induction to Peptide-Conjugated Vaccine in Squalene Emulsion Contrast With Aluminum Hydroxide Formulation. Front Immunol. 2022 Apr 7;13:848571. doi: https://doi.org/10.3389/fimmu.2022.848571. PMID: 35464449; PMCID: PMC9021396.
Sandberg, A., Berenjeno-Correa, E., Rodriguez, R.C. et al. Aβ42 oligomer-specific antibody ALZ-201 reduces the neurotoxicity of Alzheimer’s disease brain extracts. Alz Res Therapy 14, 196 (2022). https://doi.org/10.1186/s13195-022-01141-1
Villatoro, A., Cuminetti, V., Bernal, A. et al. Endogenous IL-1 receptor antagonist restricts healthy and malignant myeloproliferation. Nat Commun 14, 12 (2023). https://doi.org/10.1038/s41467-022-35700-9
Michel Cogne, Natsuko UEDA, Marine CAHEN et al. Single-hit genome editing in B cells to redirect their specificity toward tumor antigens, 06 May 2024, PREPRINT (Version 1) available at Research Square https://doi.org/10.21203/rs.3.rs-4317490/v1
Ueda, N., Cahen, M., Leonard, J. et al. Single-hit genome editing optimized for maturation in B cells redirects their specificity toward tumor antigens. Sci Rep 14, 22432 (2024). https://doi.org/10.1038/s41598-024-74005-3

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XtenCHO Race Expression medium

Référence NB-21-23859

Marque : Neo Biotech