STAT3 pTyr705 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 9E12]

Specifications

Product Data
Clone Name	9E12
Applications	ELISA, IF, IHC, IP, WB
Recommended Dilution	Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.  Included Positive Control: Cell lysate from pervanadate treated  HepG2 cells. ELISA: 0.05 µg/ml. Immunoprecipitation: 1-10 µg per 106 pervanadate treated HepG2 cells. Immunocytochemistry: 1-10 µg/ml. Immunohistochemistry.
Reactivities	Canine, Human, Mouse
Host	Mouse
Isotype	IgG1
Clonality	Monoclonal
Immunogen	Synthetic phosphopeptide conjuagted to KLH
Specificity	This antibody specifically recognizes STAT3 phosphorylated at Tyr 705. The antibody does not cross react with the non-phosphorylated form of STAT3 nor with unrelated tyrosine-phosphorylated proteins.
Formulation	PBS with 0.09% Sodium Azide, PEG and Sucrose Label: Biotin State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Size Exclusion Chromatography
Conjugation	Biotin
Storage	Store the antibody (Aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Stability	Shelf life: one year from despatch.
Gene Name	signal transducer and activator of transcription 3
Database Link	Entrez Gene 6774 Human UniProt ID P40763
Background	The STAT proteins serve as both cytoplasmic signal transducers and nuclear activators of transcription. STATs are mediators involved in cytokine signalling. In response to a specific cytokine signal, STAT proteins are phosphorylated on conserved tyrosine residues. Phosphorylated STAT proteins dimerize via their SH2 domains and move to the nucleus. The STAT dimers bind to specific DNA elements resulting in transcriptional regulation of downstream target genes. STAT3 is activated by tyrosine phosphorylation at residue Tyr 705 in cells treated with interleukin 6 or EGF. Activated STAT3 can bind to DNA either as homodimer or as heterodimer with STAT1.
Synonyms	STAT-3, Acute-phase response factor, APRF
Note	Protocol: Positive Control Provided: HepG2 pervanadate treated. Description: Cell lysate from HepG2 cells. Serum starved cells werer treated form15 min with pervanadate. Format: Lyophilized cell lysate from HepG2 cells. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80.000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.
Reference Data