CD5 antibody [53-7.3] (APC-Cy7)

Référence GTX01465-15-100ug

Conditionnement : 100ug

Marque : Genetex

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Anti-CD5 antibody [53-7.3] (APC-Cy7) used in Flow cytometry (FACS). GTX01465-15

Host

Rat

Clonality

Monoclonal

Clone Name

53-7.3

Isotype

IgG2a

Application

FACS

Reactivity

Mouse
Package
100 μg
View product citations for antibody GTX01465-15 on CiteAb
PRODUCT

Summary

The 53-7.3 monoclonal antibody is specific for mouse CD5, a 67 kDa protein that is expressed on most thymocytes, mature T cells and the B-1 subset of B cells. CD5 is a member of the scavenger receptor cysteine-rich protein superfamily and is a ligand for the c-type lectin CD72. CD5 is also known as Leu-1 and functions to modulate antigen receptor signaling on T and B cells.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Allophycocyanin-Cyanine7 (APC-Cy7)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Cell membrane

Database

DATA IMAGES

Anti-CD5 antibody [53-7.3] (APC-Cy7) used in Flow cytometry (FACS). GTX01465-15

GTX01465-15 FACS Image

FACS analysis of mouse C57Bl/6 splenocytes using GTX01465-15 CD5 antibody [53-7.3] (APC-Cy7).
Solid lone : primary antibody
Dashed line : isotype control
antibody amount : 0.25 μg (5 μl)

Clone Reference

1998; CD5 expression is developmentally regulated by T cell receptor (TCR) signals and TCR avidity.
1998; A role for CD5 in cognate interactions between T cells and B cells, and identification of a novel ligand for CD5.
1996; CD5-mediated negative regulation of antigen receptor-induced growth signals in B-1 B cells.
1995; A role for CD5 in TCR-mediated signal transduction and thymocyte selection.
1983; The "Ly-1 B" cell subpopulation in normal immunodefective, and autoimmune mice.
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REVIEW

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Clone Reference
1998; CD5 expression is developmentally regulated by T cell receptor (TCR) signals and TCR avidity.
1998; A role for CD5 in cognate interactions between T cells and B cells, and identification of a novel ligand for CD5.
1996; CD5-mediated negative regulation of antigen receptor-induced growth signals in B-1 B cells.
1995; A role for CD5 in TCR-mediated signal transduction and thymocyte selection.
1983; The "Ly-1 B" cell subpopulation in normal immunodefective, and autoimmune mice.
1980; T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views.
SDS
Sodium Azide.pdf