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> ADAM22 (cytoplasmic) Antibody

ADAM22 (cytoplasmic) Antibody

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Référence ASM10245-P594-100

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  • ICC/IF - ADAM22 (cytoplasmic) Antibody ASM10245
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-ADAM22 (cytoplasmic) Monoclonal Antibody, Clone S46-30 (ASM10245). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-ADAM22 (cytoplasmic) Monoclonal Antibody (ASM10245) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasm. Magnification: 60X.
  • WB - ADAM22 (cytoplasmic) Antibody ASM10245
    Western Blot analysis of Rat brain membrane lysate showing detection of ADAM22 protein using Mouse Anti-ADAM22 Monoclonal Antibody, Clone S46-30 (ASM10245). Primary Antibody: Mouse Anti-ADAM22 Monoclonal Antibody (ASM10245) at 1:100.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC/IF, IP
Primary Accession Q9R1V6
Other Accession NP_001007221.1
Host Mouse
Isotype IgG2B
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Description Mouse Anti-Mouse ADAM22 (cytoplasmic) Monoclonal IgG2B
Target/Specificity Detects the cytoplasmic domain of ADAM22 ~90kDa. Weak human detection.
Other Names MDC2 Antibody, Disintegrin and metalloproteinase domain-containing protein 22 Antibody, Metalloproteinase disintegrin ADAM22-3 Antibody, Metalloproteinase-like disintegrin-like and cysteine-rich protein 2 Antibody, ADAM 22 Antibody, ADAM metallopeptidase domain 22 Antibody, MGC149832 Antibody
Clone Names S46-30
Immunogen Fusion protein amino acids 757-857 (cytoplasmic region) of mouse ADAM22
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-411 was sufficient for detection ofADAM22 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Membrane