Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.
 
> HSP90 alpha Antibody

HSP90 alpha Antibody

Imprimer Imprimer
Identifiez-vous

Référence ASM10067-P594-200

Conditionnement : Onrequest

Marque : Abcepta

Contactez votre distributeur local :


Téléphone : +1 850 650 7790

 
  • ICC/IF - HSP90 alpha Antibody ASM10067
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone 2G5.G3 (ASM10067). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody (ASM10067) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.
  • WB - HSP90 alpha Antibody ASM10067
    Western Blot analysis of Rat tissue lysate showing detection of Hsp90 alpha protein using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone 2G5.G3 (ASM10067). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody (ASM10067) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC/IF, IP, E
Primary Accession P07900
Other Accession NP_001017963.2
Host Mouse
Isotype IgG
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Description Mouse Anti-Human HSP90 alpha Monoclonal IgG
Target/Specificity Detects ~90kDa. HSP90α-specific (>96% α-specific by ELISA)
Other Names HSP86 Antibody, HSP89A Antibody, HSP90AA1 Antibody, HSP90Alpha Antibody, HSPC1 Antibody, HSPCA Antibody, HSPCAL3 Antibody
Clone Names 2G5.G3
Immunogen Human HSP90alpha
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.2, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 0.5 µg/ml of SMC-147 was sufficient for detection of HSP90alpha in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm | Melanosome