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> HSP90 complex Antibody

HSP90 complex Antibody

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Référence ASM10014-P594-200

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  • ICC/IF - HSP90 complex Antibody ASM10014
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp90 complex Monoclonal Antibody, Clone 8D3 (ASM10014). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsp90 complex Monoclonal Antibody (ASM10014) at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Mouse (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp90 complex Antibody. (C) Composite.
  • IP - HSP90 complex Antibody ASM10014
    Immunoprecipitation analysis using Mouse Anti-Hsp90 complex Monoclonal Antibody, Clone 8D3 (ASM10014). Tissue: reticulocyte lysate. Species: Rabbit. Primary Antibody: Mouse Anti-Hsp90 complex Monoclonal Antibody (ASM10014) at 1:1000.
  • ICC/IF - HSP90 complex Antibody ASM10014
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp90 complex Monoclonal Antibody, Clone 8D3 (ASM10014). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsp90 complex Monoclonal Antibody (ASM10014) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Mouse (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp90 complex Antibody. (C) Composite.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IP, ICC/IF
Primary Accession P08238
Other Accession NP_031381.2
Host Mouse
Isotype IgM
Reactivity Human, Mouse, Rat, Rabbit
Clonality Monoclonal
Description Mouse Anti-Human HSP90 complex Monoclonal IgM
Target/Specificity Detects 90kDa. Co-immunoprecipitates HSP90 complexes, including HSP70, Hop, Ah receptors, glucocorticoid receptors, heme-regulated eukaryotic initiation factor 2α (eIF-2α) kinase (HRI).
Other Names HSP84 Antibody, HSP90 Antibody, HSP90 beta Antibody, HSP90B Antibody, HSPC2 Antibody, HSPCB Antibody, Heat shock protein HSP 90-beta Antibody, HSP 90 Antibody, Heat shock 84 kDa Antibody, HSP 84 Antibody, HSP84 Antibody, HSP90AB1 Antibody, HSP90B Antibody, HSPC2 Antibody, HSPCB Antibody
Clone Names 8D3
Immunogen Ah receptor (Aryl hydrocarbon receptor)
Purification PEG Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis Goat anti-mouse IgM was used to bind 25 µl of protein G-Sepharose. SMC-109 IgM from 0.5 ml of high speed supernatant medium was loaded onto the IgG resin and incubated with 100 µl of rabbit reticulocyte lysate for 30 min. at 30C. After washing (4X1 ml), bound proteins were resolved on SDS PAGE, including HSP90, HSP70 and Hop.
Cellular Localization Cytoplasm | Melanosome