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> Tsp23 Antibody

Tsp23 Antibody

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Référence ASM10142-P594-100

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  • IHC - Tsp23 Antibody ASM10142
    Immunohistochemistry analysis using Mouse Anti-Tsp23 Monoclonal Antibody, Clone TSp232A (ASM10142). Tissue: Heart Skeletal Muscle. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Tsp23 Monoclonal Antibody (ASM10142) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT.
  • WB - Tsp23 Antibody ASM10142
    Western Blot analysis of Human Heat Shocked HeLa cell lysates showing detection of Tsp23 protein using Mouse Anti-Tsp23 Monoclonal Antibody, Clone TSp232A (ASM10142). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Tsp23 Monoclonal Antibody (ASM10142) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession B9A003
Other Accession NP_001136126.1
Host Mouse
Isotype IgG1 Kappa
Reactivity Human, Mouse
Clonality Monoclonal
Description Mouse Anti-Human Tsp23 Monoclonal IgG1 Kappa
Target/Specificity Detects ~19kDa. Detects Tsp23, no cross-reactivity to p23.
Other Names Transcript similar p23 Antibody, AARSD1 Antibody, AASD1_HUMAN Antibody, Alanyl-tRNA editing protein Aarsd1 Antibody, Alanyl-tRNA synthetase domain containing 1 Antibody, Alanyl-tRNA synthetase domain-containing protein 1 Antibody, MGC2744 Antibody, OTTHUMP00000204017 Antibody, OTTHUMP00000204019 Antibody, OTTHUMP00000204020 Antibody
Clone Names Tsp232A
Immunogen Recombinant tsp23
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-194 was sufficient for detection of tsp23 in 20 µg of transiently transfected Hela cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm