ASM10186-STR-100 | KCNQ2 Antibody Clinisciences

Immunohistochemistry analysis using Mouse Anti-KCNQ2 Monoclonal Antibody, Clone S26A-23 (ASM10186). Tissue: frozen brain section. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-KCNQ2 Monoclonal Antibody (ASM10186) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT.
Western Blot analysis of Human, hamster HEK and CHO cell lysates showing detection of KCNQ2 protein using Mouse Anti-KCNQ2 Monoclonal Antibody, Clone S26A-23 (ASM10186). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-KCNQ2 Monoclonal Antibody (ASM10186) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. KCNQ2 overexpressed.
Immunohistochemistry analysis using Mouse Anti-KCNQ2 Monoclonal Antibody, Clone S26A-23 (ASM10186). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-KCNQ2 Monoclonal Antibody (ASM10186) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immunoelectronmicroscopy EIA=Enzyme Immunoassay	WB, IHC, IP, AM, ICC/IF
Primary Accession	O43526
Other Accession	NP_004509.2
Host	Mouse
Isotype	IgG1
Reactivity	Human, Mouse, Rat, Hamster
Clonality	Monoclonal
Description	Mouse Anti-Human KCNQ2 Monoclonal IgG1
Target/Specificity	Detects ~95kDa.
Other Names	BFNC Antibody, EBN1 Antibody, ENB1 Antibody, HNSPC Antibody, KCNA11 Antibody, KQT like 2 Antibody, KV7.2 Antibody, KVEBN1. KvLQT2 Antibody, voltage gated potassium channel subunit Kv7.2 Antibody
Clone Names	S26A-23
Immunogen	Fusion protein amino acids 1-59 of human KCNQ2
Purification	Protein G Purified
Storage	-20ºC
Storage Buffer	PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature	Blue Ice or 4ºC
Certificate of Analysis	1 µg/ml of SMC-308 was sufficient for detection of KCNQ2 in 10 µg of COS-1 cell lysate transiently expressing KCNQ2 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization	Membrane

KCNQ2 Antibody