TrpC5 Antibody

Référence ASM10220-STR-100

Conditionnement : Onrequest

Marque : Abcepta

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  • IHC - TrpC5 Antibody ASM10220
    Immunohistochemistry analysis using Mouse Anti-TrpC5 Monoclonal Antibody, Clone S67-15 (ASM10220). Tissue: Brain Slice. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-TrpC5 Monoclonal Antibody (ASM10220) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT. Localization: Nuclear staining. Magnification: 10X.
  • WB - TrpC5 Antibody ASM10220
    Western Blot analysis of Rat Brain Membrane showing detection of ~110 kDa TrpC5 protein using Mouse Anti-TrpC5 Monoclonal Antibody, Clone S67-15 (ASM10220). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain Membrane cell lysate. Load: 20 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-TrpC5 Monoclonal Antibody (ASM10220) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:100 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~110 kDa. Other Band(s): 160kDa, 60kDa.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IP
Primary Accession Q9UL62
Other Accession NP_036603.1
Host Mouse
Isotype IgG2b
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Description Mouse Anti-Human TrpC5 Monoclonal IgG2b
Target/Specificity Detects ~110kDa.
Other Names Htrp5 Antibody, Short transient receptor potential channel 5 Antibody, TRP5 Antibody
Clone Names S67-15
Immunogen Synthetic peptide amino acids 827-845 of human TrpC5 (also known as short transient receptor potential channel 5, and Htrp5)
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-344 was sufficient for detection of TrpC5 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Membrane