Anti-Phospho-IRS1-Ser636 antibody (570-650)

Référence NB-22-0308-200ul

Conditionnement : 200µg

Marque : Neo Biotech

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Téléphone : +1 850 650 7790

  • Western blot analysis of lysates from 293 cells and COLO205 cells, using IRS-1 (Phospho-Ser636) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunofluorescence analysis of MCF7 cells, using IRS-1 (Phospho-Ser636) Antibody. The picture on the right is blocked with the phospho peptide.
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IRS-1 (Phospho-Ser636) Antibody. The picture on the right is blocked with the phospho peptide.
  • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Western blot analysis of 293T 3T3 lysis using Phospho-IRS-1 (S636) antibody. Antibody was diluted at 1:2000
  • Western blot analysis of RAW using Phospho-IRS-1 (S636) Polyclonal Antibody diluted at 1:2000
  • Western blot analysis of SH-SY5Y cells using Phospho-IRS-1 (S636) Polyclonal Antibody diluted at 1:2000
  • Western blot analysis of various cells using Phospho-IRS-1 (S636) Polyclonal Antibody diluted at 1:2000

General Info

Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Insulin receptor substrate 1-Ser636 (603-652 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol: IRS1
Gene ID: 3667
Uniprot ID: IRS1_HUMAN
Immunogen Region: 603-652 aa
Specificity: Phospho-IRS-1 (S636) Polyclonal Antibody detects endogenous levels of IRS-1 protein only when phosphorylated at S636.
Immunogen: The antiserum was produced against synthesized peptide derived from the human IRS-1 around the phosphorylation site of Ser636 at the amino acid range 603-652

Description

Function May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
Protein Name Insulin Receptor Substrate 1
Irs-1
Database Links Reactome: R-HSA-109704
Reactome: R-HSA-112399
Reactome: R-HSA-112412
Reactome: R-HSA-1257604
Reactome: R-HSA-1266695
Reactome: R-HSA-198203
Reactome: R-HSA-201556
Reactome: R-HSA-2219530
Reactome: R-HSA-2428928
Reactome: R-HSA-2586552
Reactome: R-HSA-5673001
Reactome: R-HSA-6811558
Reactome: R-HSA-74713
Reactome: R-HSA-74749
Reactome: R-HSA-9603381
Reactome: R-HSA-9725370
Reactome: R-HSA-982772
Alternative Antibody Names Anti-Insulin Receptor Substrate 1 antibody
Anti-Irs-1 antibody
Anti-IRS1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance