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> Anti-Cleaved-CASP6-D162 antibody (120-200 Internal)

Anti-Cleaved-CASP6-D162 antibody (120-200 Internal)

Imprimer Imprimer
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Référence NB-22-0007

Conditionnement : 100µg

Marque : Neo Biotech

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Téléphone : +1 850 650 7790

  • Western blot analysis of lysates from HeLa cells, treated with Etoposide 25uM 60', using Caspase 6 (Cleaved-Asp162) Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of HeLa cells using Cleaved-Caspase-6 p18 (D162) Polyclonal Antibody diluted at 1：1000
  • Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Caspase 6 (Cleaved-Asp162) Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using Cleaved-Caspase-6 p18 (D162) Polyclonal Antibody diluted at 1：1000

General Info

Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Cleaved-CASP6-D162 (144-193 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Immunogen Region: 144-193 aa
Specificity: Cleaved-Caspase-6 p18 (D162) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-6 p18 protein resulting from cleavage adjacent to D162.
Immunogen: The antiserum was produced against synthesized peptide derived from mouse Caspase 6 at the amino acid range 144-193

Description

Function Cysteine protease that plays essential roles in programmed cell death, axonal degeneration, development and innate immunity. During apoptosis, localizes in the nucleus and cleaves the nuclear structural protein NUMA1 and lamin A/LMNA thereby inducing nuclear shrinkage and fragmentation. Furthermore, cleaves many transcription factors such as NF-kappa-B and cAMP response element-binding protein/CREBBP. Plays an essential role in axon degeneration during axon pruning which is the remodeling of axons during neurogenesis but not apoptosis. Regulates B-cell programs both during early development and after antigen stimulation. In addition, promotes the ZBP1-mediated activation of programmed cell death pathways including pyroptosis, apoptosis, and necroptosis (PANoptosis) and plays an essential role in defense against viruses. Mechanistically, interacts with RIPK3 and enhances the interaction between RIPK3 and ZBP1, leading to ZBP1-mediated inflammasome activation and cell death.
Protein Name Caspase-6
Casp-6
Apoptotic Protease Mch-2 Cleaved Into - Caspase-6 Subunit P18 - Caspase-6 Subunit P11
Database Links Reactome: R-HSA-111465
Reactome: R-HSA-264870
Reactome: R-HSA-352238
Reactome: R-HSA-6803207
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Caspase-6 antibody
Anti-Casp-6 antibody
Anti-Apoptotic Protease Mch-2 Cleaved Into - Caspase-6 Subunit P18 - Caspase-6 Subunit P11 antibody
Anti-CASP6 antibody
Anti-MCH2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance