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> κ-Carrageenan [11114-20-8]

κ-Carrageenan [11114-20-8]

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Référence NB-64-78349-100mg

Conditionnement : 100mg

Marque : Neo Biotech


κ-Carrageenan

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κ-Carrageenan is a natural polymer predominantly found in red seaweeds that can serve as an effective carrier to deliver curcumin to cancer cells and induce apoptosis. It can also be used to induce paw swelling models and acts as a potential inflammatory agent that amplifies existing intestinal inflammation.

κ-Carrageenan
Other Forms of κ-Carrageenan:
CarrageenanT353279000-07-1
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Product Introduction
Bioactivity
Description
κ-Carrageenan is a natural polymer predominantly found in red seaweeds that can serve as an effective carrier to deliver curcumin to cancer cells and induce apoptosis. It can also be used to induce paw swelling models and acts as a potential inflammatory agent that amplifies existing intestinal inflammation.
In vitro
κ-Car-Curcumin (Cur) (0-500 μg/mL; 24-72 hours) effectively inhibits the growth of cancer cells at concentrations as low as 40 μg/mL[1]. The Cur-loaded κ-Car displays significant cytotoxicity, with high apoptotic activity observed in A549 lung cancer cells[1]. In addition, κ-Carrageenan (1-60 μg/mL; 0.5-24 hours) is shown to amplify LPS-induced IL-8 secretion in HT-29 cells[2]. A Cell Viability Assay on the A549 cell line with Cur concentrations ranging from 0-500 μg/mL over 24, 48, and 72 hours demonstrates dose-responsive effects, yielding significant IC 50 values of 65, 50, and 40 μg/mL for 24, 48, and 72 hours, respectively[1].
In vivo
κ-Carrageenan, administered orally at dosages ranging from 1.7 to 41.7 mg/kg for 1 week prior to Citrobacter freundii DBS100 treatment, markedly enhances LPS-induced inflammation via the Bcl10-NF-κB pathway. This action is evident from the exacerbation of C. freundii DBS100-induced colitis in mice, particularly through the increased induction of TLR4 and NF-κB in the intestinal mucosa of the affected animals. Moreover, κ-Carrageenan intensifies TNBS-induced intestinal inflammation, a process possibly linked to oxidative stress alongside the activation of both the TLR4-NF-κB and MAPK/ERK1/2 pathways. This observation was made in studies utilizing both male and female NIH (s) mice, with the compound being orally administered in low, medium, and high doses, specifically 1.7 mg/kg, 8.3 mg/kg, and 41.7 mg/kg, respectively. The results indicate a heightened induction of TLR4 and NF-κB pathways in the intestinal mucosa of mice infected with C. freundii DBS100.
Disease Modeling Protocol
Foot swelling inflammation model
  • Modeling Mechanism：

    Subcutaneous injection of κ-Carrageenan activates the local inflammatory response, induces mast cell degranulation and neutrophil infiltration, and releases inflammatory mediators such as histamine, serotonin, and bradykinin. At the same time, it activates the NF-κB signaling pathway, promotes the production of pro-inflammatory factors such as TNF-α, IL-6, PGE2, and COX-2, as well as reactive oxygen species (ROS), and causes interstitial edema, vasodilation, and structural damage in the foot tissues, forming a typical acute inflammatory swelling phenotype.

  • Related Products：

    κ-Carrageenan (T38499)

  • Modeling Method：

    Experimental Subject：Rats, Sprague–Dawley, Male, 180–200g, 8–10 weeks old

    Dosage and Administration Route：100 μg / paw κ-Carrageenan (dissolved in 0.9% saline, final volume 0.1 mL) , Right hind paw subplantar injection

    Dosing Frequency and Duration Model：Single dose

  • Validation：

    Clinical phenotype: Foot swelling gradually worsened after drug administration, reaching its peak at 2 hours. Foot volume was measured using a plethysmometer, and the swelling rate was ≥30% compared to the control group (injected with 0.1 mL of normal saline). Histological indicators: HE staining of foot tissue showed interstitial edema, inflammatory exudation, vascular damage, and erythrocyte exudation, with disordered tissue arrangement. Molecular indicators: Serum levels of TNF-α, IL-6, PGE2, and COX-2 were significantly elevated (P<0.001), consistent with the characteristics of acute inflammatory factor release. Specificity verification: The positive control drug indomethacin (INDO, 5 mg/kg orally) significantly inhibited foot swelling and inflammatory factor levels, confirming the effectiveness of the model.

*Precautions： All rats were sacrificed 2 hours after modeling, and foot tissue and serum were collected for testing.

*References：Xiang L,et,al. Ethanol extract of Paridis rhizoma attenuates carrageenan-induced paw swelling in rats by inhibiting the production of inflammatory factors. BMC Complement Med Ther. 2023 Dec 4;23(1):437.

Chemical Properties
Cas No.11114-20-8
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.