2.5% Trypsin-EDTA Solution 10X
Référence sc-391060
Conditionnement : 100ml
Marque : Santa Cruz Biotechnology
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2.5% Trypsin-EDTA Solution 10X (CAS 9002-07-7 (solid))
Noms alternatifs:
2.5% Trypsin-EDTA Solution 10X is also known as Tryptase.
Application:
2.5% Trypsin-EDTA Solution 10X is an agent used to disengage adherent cells from tissue culture plates.
Numéro CAS:
9002-07-7 (solid)
Pour la Recherche Uniquement. Non conforme pour le Diagnostic ou pour une Utilisation Thérapeutique.
* Consulter le Certificat d'Analyses pour les données spécifiques à un lot (incluant la teneur en eau).
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Description
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Données de Sécurité
SDS & Certificat d’Analyses
2.5% Trypsin-EDTA Solution 10X is a concentrated solution that can be diluted to the 1x working concentation of 0.25% Trypsin-EDTA using an appropriate buffer. This 2.5% Trypsin-EDTA Solution 10X is used to release adherent cells from tissue culture plates. Due to its digestive strength, 2.5% Trypsin-EDTA Solution 10X is also widely used for cell dissociation, routine cell culture passaging, and primary tissue dissociation.
References:
- [Long-term in vitro culture of the fibroblasts from the deep partial thickness burn wound in burn patients]. | Li, YL., et al. 2003. Zhonghua Shao Shang Za Zhi. 19: 35-7. PMID: 12678974
- Increased proteolysis of collagen in an in vitro tensile overload tendon model. | Willett, TL., et al. 2007. Ann Biomed Eng. 35: 1961-72. PMID: 17763961
- Different methods of detaching adherent cells significantly affect the detection of TRAIL receptors. | Zhang, B., et al. 2012. Tumori. 98: 800-3. PMID: 23389369
- Biophysical characterization of low-frequency ultrasound interaction with dental pulp stem cells. | Ghorayeb, SR., et al. 2013. J Ther Ultrasound. 1: 12. PMID: 25516801
- Atomic force microscopy in the production of a biovital skin graft based on human acellular dermal matrix produced in-house and in vitro cultured human fibroblasts. | Łabuś, W., et al. 2018. J Biomed Mater Res B Appl Biomater. 106: 726-733. PMID: 28323389
- A novel autophagy modulator 6-Bio ameliorates SNCA/ alpha -synuclein toxicity. | Suresh, SN., et al. 2017. Autophagy. 13: 1221-1234. PMID: 28350199
- Development of a new method for the preparation of an acellular allodermis, quality control and cytotoxicity testing. | Dragúová, J., et al. 2017. Cell Tissue Bank. 18: 153-166. PMID: 28405854
- Specific capture, recovery and culture of cancer cells using oriented antibody-modified polystyrene chips coated with agarose film. | Jeong, J., et al. 2018. Colloids Surf B Biointerfaces. 162: 306-315. PMID: 29220830
- A simple trypsin resistance assay for muscle and other cell fusion. | Denning, G. and Fulton, AB. 1986. J Histochem Cytochem. 34: 959-62. PMID: 3086428
- Collagen Fibrils Mechanically Contribute to Tissue Contraction in an In Vitro Wound Healing Scenario. | Brauer, E., et al. 2019. Adv Sci (Weinh). 6: 1801780. PMID: 31065517
- Estimation and in-situ detection of thorium in human liver cell culture by arsenazo-III based colorimetric assay. | Yadav, R., et al. 2020. Biometals. 33: 75-85. PMID: 31897857
- Establishing Sustainable Cell Lines of a Coral, Acropora tenuis. | Kawamura, K., et al. 2021. Mar Biotechnol (NY). 23: 373-388. PMID: 33899125
- Morphologic changes in rat urothelial cells during carcinogenesis: I. Histologic and cytologic changes. | King, EB., et al. 1984. Cytometry. 5: 447-53. PMID: 6489059
- Primary culture of bovine gall bladder epithelial cells. | Plevris, JN., et al. 1993. Gut. 34: 1612-5. PMID: 8244152
Formulation :
Solution in HBSS. 2.5% trypsin, 0.2% EDTA solution in saline, lacking phenol red
État Physique :
Liquid
STOCKAGE :
Store at -20° C
Pour la Recherche Uniquement. Non conforme pour le Diagnostic ou pour une Utilisation Thérapeutique.
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SAMPLE Certificate of Analysis (COA)
2.5% Trypsin-EDTA Solution 10X | CAS 9002-07-7 (solid) SAMPLE Certificate of AnalysisCERTIFICAT D'ANALYSE
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Numéro de Lot
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SAMPLE Certificate of Analysis (COA)
Catalog Number:sc-391060
Lot Number:SAMPLE
Product Name:2.5% Trypsin-EDTA Solution 10X
| Test | Specification | Results |
|---|---|---|
| Appearance | Clear liquid | |
| pH | 7.5 | |
| Sterility | Filtered with 0.22 µm filter |
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