2-Arachidonoylglycerol ELISA Kit
Référence OKCD02248
Conditionnement : 96Wells
Marque : Aviva Systems Biology
Datasheets/Manuals | Printable datasheet for 2-Arachidonoylglycerol ELISA Kit (OKCD02248) |
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COA Datasheet | Click Hereto download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
Predicted Species Reactivity | All Species | ||||||||||||||||||||||
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Application | ELISA-Comp | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 2h | ||||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 2-AG has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 2-AG analogues and unlabeled antigen (Standards or samples) with the pre-coated antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 2-AG in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 2-AG in the sample. | ||||||||||||||||||||||
ELISA Kit Range | 3.70-300ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 2-Arachidonoylglycerol (2-AG) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 2-Arachidonoylglycerol (2-AG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Additional Information | CAS: 53847-30-6 PubChem: 5282280 | ||||||||||||||||||||||
Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||||||||
Sensitivity | 1.41 ng/mL | ||||||||||||||||||||||
Assay Info | Assay Methodology: Competitive Inhibition Immunoassay |
Gene Symbol | 2-AG |
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Alias Symbols | 2-arachidonoylglycerol; 2-Arachidonylglycerol; 2-AG; 2-Arachidonyl-glycerol; 2-Arachidonoyl-glycerol; 2-Arachidonoyl Glycerol |
Protein Name | 2-Arachidonoylglycerol |