Advillin antibody
Référence orb666108-100ul
Conditionnement : 100ul
Marque : Biorbyt
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Téléphone : +1 850 650 7790
Item 1 of 2
Item 1 of 2
Advillin antibody
Catalog Number: orb666108
| Catalog Number | orb666108 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to Advillin |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IF, WB |
| Reactivity | Human, Mouse, Rat |
| Immunogen | Recombinant fusion protein of human Advillin. The exact sequence is proprietary. |
| Dilution range | WB: 1-500-2000 |
| Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Conjugation | Unconjugated |
| Target | AVIL |
| Entrez | 11567, 10677 |
| UniProt ID | O75366, Q9WU06, O88398 |
| Source | Rabbit |
| Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles. |
| Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Alternative names | Anti-Advillin antibody, anti-p92 antibody |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of Advillin expression in HT29 (A), mouse brain (B), rat testis (C), rat brain (D) whole cell lysates. (Predicted band size: 91; 92 kD; Observed band size: 105; 115 kD)
Immunofluorescent analysis of Advillin staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).