Alexa Fluor® 488 - FluoroNanogold™ streptavidin
Référence N-7216-1ML
Conditionnement : 1.0mL
Marque : Nanoprobes
Contactez votre distributeur local :
Téléphone : +1 850 650 7790
Fluorescent labels PLUS Nanogold® - covalently bound
Make your target visible in almost any microscope !
See your target's fluorescent label as before
PLUS the Nanogold label in light microscopes, even EM
Put your targets back into their cellular context!
Correlate between confocal, light microscopy and EM
- Available in convenient conjugates to secondary antibodies (or biotin/streptavidin)
- Uses standard secondary antibody labeling protocols
- Easily grow the Nanogold® particle to any size after labeling
with our Gold and Silver Enhancers
-for low-mag applications, or even to the naked eye
"FNG [FluoroNanogold™] is a probe containing two different markers; it opens the possibility of imaging the same sample at both the optical and the EM level. Thus, it is easy to perform a multimodal investigation, either on different cells or on the same cell visualized by correlative microscopy."
Cheutin, T.; Sauvage, C.; Tchélidzé, P, O'Donohue, M. F.; Kaplan, H.; Beorchia, A., and Ploton, D.: Visualizing macromolecules with FluoroNanogold: from photon microscopy to electron tomography. Methods Cell Biol., 79, 559–574 (2007). [This is an entire chapter on FluoroNanogold, providing a detailed and well illustrated write-up of techniques for combined confocal fluorescence microscopic labeling and electron tomography.]
Learn more about our FluoroNanogold™ products...
Available in convenient conjugates to secondary antibodies, or streptavidin:
- Features and applications
- Specific properties of conjugates:
- Fluorescein FluoroNanogold™
- Alexa Fluor®* 488 FluoroNanogold™
- Alexa Fluor®* 594 FluoroNanogold™
- Alexa Fluor®* 546 FluoroNanogold™
Alexa Fluor® 647 FluoroNanogold™ for super-resolution!
- Custom FluoroNanogold™ Conjugates
- Featured paper: Sato et al. (2013) used FluoroNanogold™ to explore the mechanism behind cellular dynamics, using correlative microscopy with fluorescence and the new Atmospheric SEM (ASEM). Check out their great images!
- References: Papers published that use FluoroNanogold™
- Instructions and Protocols
- Technical Help
- MSDS
By combining gold and fluorescence into one immunoprobe, the same specimen may be imaged using both fluorescence microscopy (e.g., with a confocal microscope) and at the ultrastructural level by electron microscopy.
FluoroNanogold™is now available with Alexa Fluor®* 647, 546, 488 or 594, giving you the benefits of brighter fluorescence, reduced photobleaching, and compatibility with a wider pH range, or in its original formulation with fluorescein as the fluorophore.
Unlike colloidal golds which quench fluorescence, tiny Nanogold® lets the fluorescence shine through. All components are covalently attached to ensure stability and long shelf life. Fab' antibody fragments are much smaller probes than IgG conjugates, and have shown excellent penetration into cells and nuclei. FluoroNanogold™conjugates are chromatographically purified to eliminate any aggregates, free gold or unattached fluorescent molecules.
- Unprecedented correlation between fluorescence and EM data.
- Single labeling procedure means less chance for specimen perturbation.
- Choice of new fluorophores for brighter fluorescence, lower background and multicolor labeling.
- Same excellent penetration as found with Nanogold®: much better than 5 and 10 nm colloidal gold probes.
- FluoroNanogold™ probes are smaller than IgG conjugates (we use Fab' fragments).
- Covalent coupling of both labels gives stability and long shelf life.
- Conjugates are stable and fluorescent at a wide range of pH and ionic strengths.
Applications
- Correlative fluorescence and electron microscopy (References)
- Check your labeling by fluorescence before processing for EM
- Monitor a dynamic process by fluorescence to determine when to fix and process for EM.
- Differentiate different targets using different fluorophores.
| Alexa Fluor®* 647 FluoroNanogold™ 2-in-1 immunolabeling for Super-Res! | |
| Simultaneously label for Super-Res Each secondary antibody / Fab' includes TWO labels
Finally-- Put your Super-Res images into cellular context!
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| Fluorescent labeling of Alexa Fluor® 647 and Nanogold® - Fab' tertiary probe. The specimen is a slide from the NOVA Lite ANA HEp-2 test, an indirect immunofluorescent test system for the screening and semi-quantitative determination of anti-nuclear antibodies (ANA) in human serum (see ). The slide was stained using positive pattern control human sera, a Mouse anti-Human secondary antiboidy, and combined Alexa Fluor® 647 and Nanogold® - Fab' tertiary probe. Specimens were washed with PBS (30 minutes) between each step, then blocked by the addition of 7 % nonfat dried milk to the tertiary antibody solution (original magnification 400 X). |
Alexa Fluor® 647 FluoroNanogold™
| Alexa Fluor® 647 FluoroNanogold™ |
Alexa Fluor®* 488 FluoroNanogold™
Our new Alexa Fluor® FluoroNanogold™ conjugates will give you greatly enhanced performance compared with conventional fluorophores. Benefit from greatly improved fluorescence properties, combined with a new level of freedom from background and non-specific binding.- Increased fluorescence signal and higher quantum yield - ideal for scarce targets or dynamic systems where exposure needs to be restricted.
- Fluorescence remains high and consistent across wider pH range.
- Improved solubility means reduced non-specific interactions, lower background and higher signal-to-noise ratios.
- Uses fluorescein filter sets.
- Available in 1 mL or affordable 0.5 mL sizes.
Left: Structure of Alexa Fluor® 488 and Nanogold® - Fab', showing covalent attachment of components.
Right: Fluorescent staining obtained using combined combined Alexa Fluor® 488 and Nanogold® - Fab' tertiary probe. The specimen is a slide from the NOVA Lite ANA HEp-2 test, an indirect immunofluorescent test system for the screening and semi-quantitative determination of anti-nuclear antibodies (ANA) in human serum (see ). The slide was stained using positive pattern control human sera, a Mouse anti-Human secondary antiboidy, and combined Alexa Fluor® 488 and Nanogold® - Fab' tertiary probe. Specimens were washed with PBS (30 minutes) between each step, then blocked by the addition of 7 % nonfat dried milk to the tertiary antibody solution (original magnification 400 X).
Alexa Fluor® 488 FluoroNanogold™-Fab'
| Alexa Fluor® 488 FluoroNanogold™-Streptavidin
|
Product information (PDF)
Alexa Fluor®* 546 FluoroNanogold™
Our new Alexa Fluor® FluoroNanogold™ conjugates will give you greatly enhanced performance compared with conventional fluorophores. Benefit from greatly improved fluorescence properties, combined with a new level of freedom from background and non-specific binding. - Increased fluorescence signal and higher quantum yield - ideal for scarce targets or dynamic systems where exposure needs to be restricted.
- Fluorescence remains high and consistent across wider pH range.
- Improved solubility means reduced non-specific interactions, lower background and higher signal-to-noise ratios.
- Uses fluorescein filter sets.
- Available in 1 mL or affordable 0.5 mL sizes.
Alexa Fluor® 546 FluoroNanogold™-Fab'
| Alexa Fluor® 546 FluoroNanogold™-Streptavidin
|
Alexa Fluor®* 594 FluoroNanogold™
Our Alexa Fluor®* 594 FluoroNanogold™conjugates offer an a second fluorophore, enabling the labeling of more than one target within a specimen with the brightness and photostability of the Alexa Fluor® reagents.- Use for multiple labeling: distinguish a FluoroNanogold™-labeled target from a second target labeled with fluorescein, Alexa Fluor® 488, green fluorescent protein, or other fluorophores.
- Uses Texas Red filter sets.
- Available in 1 mL or affordable 0.5 mL sizes.
Localization of caveolin-1a in ultrathin cryosection of human placenta using a new FNG; caveolin 1 alpha is primarily located to caveolae in placental endothelial cells. One-to-one correspondence is found between fluorescent spots and caveola labeled with gold particles (right). Ultrathin cryosections collected on formvar film-coated nickel EM grids were incubated with chicken anti-human caveolin-1a IgY for 30 min at 37°C, then with biotinylated goat anti-chicken F(ab)2 (13 mg/ml) for 30 min at 37°C, then stained with ALEXA-594 FluoroNanogold™-Streptavidin (1:50 dilution) for 30 min at room temperature. Non-specific sites on cryosections were blocked with 1% milk - 5% fetal bovine serum-PBS for 30 minutes at room temperature (figure courtesy of T. Takizawa, Ohio State University, Columbus, OH).
Structure of Alexa Fluor®* 594 FluoroNanogold™Fab' and Streptavidin conjugates, showing covalent attachment.
Alexa Fluor® 594 FluoroNanogold™-Fab'
| Alexa Fluor® 594 FluoroNanogold™-Streptavidin
|
Fluorescein FluoroNanogold™
Fluorescein is the most widely used fluorophore; fluorescein FluoroNanogold™conjugates combine fluorescein and Nanogold® in a single convenient, stable probe.
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FluoroNanogold™Staining of Microtubules
| Immunolocalization of microtubules with Fluorescein FluoroNanogold™-Fab' in the same human monocyte visualized by various microscopies: (A) Fluorescence; (B) Phase; (C) DIC, and (D) Electron Microscopy (with silver enhancement). (Micrographs courtesy of Dr. J. M. Robinson and Dr. D. Vandré, Ohio State University). |
Fluorescein FluoroNanogold™-Fab'
| Fluorescein FluoroNanogold™-Streptavidin
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Custom Labeling
We can also create custom conjugates, using your own primary antibody (IgG or Fab'), proteins, peptides, lectins, and other molecules. Please use our Custom Synthesis Form to let us know your needs, or drop us a line by phone or email at info@clinisciences.com.
* Alexa Fluor is a registered trademark of Life Technologies, Inc.