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> Alkaline Phosphatase (Germ type) /ALPG Protein, Human, Recombinant (hFc)

Alkaline Phosphatase (Germ type) /ALPG Protein, Human, Recombinant (hFc)

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Référence NB-64-54531-500ug

Conditionnement : 500ug

Marque : Neo Biotech


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Alkaline Phosphatase (Germ type) /ALPG Protein, Human, Recombinant (hFc)

(Synonyms: PLAP-like, GCAP, ALPPL2, ALPPL, ALPG, ALP-1, Alkaline phosphatase, placental-like, Alkaline Phosphatase) Copy Product Info

Synonyms: PLAP-like, GCAP, ALPPL2, ALPPL, ALPG, ALP-1, Alkaline phosphatase, placental-like, Alkaline Phosphatase

Catalog No. TMPK-00802 Copy Product Info
Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease. Alkaline Phosphatase (Germ type) /ALPG Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 79.33 kDa and the accession number is P10696.
Other Forms of Alkaline Phosphatase (Germ type) /ALPG Protein, Human, Recombinant (hFc):
Alkaline Phosphatase Protein, Human, Recombinant (aa 23-506, His)
Alkaline Phosphatase (Germ type) /ALPG Protein, Rat, Recombinant (His)
Alkaline phosphatase (Intestinal type) /ALPI Protein, Mouse, Recombinant (His)
Alkaline phosphatase (Intestinal type) /ALPI Protein, Human, Recombinant (His & Flag)
Alkaline Phosphatase (Germ type) /ALPG Protein, Human, Recombinant (His & Avi), Biotinylated
Alkaline Phosphatase (Germ type) /ALPG Protein, Human, Recombinant (His)
Alkaline Phosphatase (Germ type) /ALPG Protein, Human, Recombinant (mFc)
Alkaline Phosphatase (Germ type) /ALPG Protein, Mouse, Recombinant (His)
Alkaline Phosphatase (Placental type) /ALPP Protein, Cynomolgus, Recombinant (His)
Alkaline Phosphatase Protein, Prevotella intermedia, Recombinant (GST & His & Myc)
Alkaline phosphatase/ALPL Protein, Rat, Recombinant (His & SUMO)
Alkaline Phosphatase, Human Placental
Alkaline Phosphatase, Porcine Kidney
Alkaline Phosphatase
Alkaline Phosphatase/ALPL Protein, Mouse, Recombinant (His)
Alkaline Phosphatase/PLAP Protein, Human, Recombinant (His)
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Introduction

Bioactivity
Bioactivity
Immobilized Human Alkaline Phosphatase (Germ Type) , hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-Alkaline Phosphatase Antibody, hFc Tag with the EC50 of 15.4ng/ml determined by ELISA.
Description
Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease. Alkaline Phosphatase (Germ type) /ALPG Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 79.33 kDa and the accession number is P10696.
Species
Human
Expression System
HEK293 Cells
TagC-hFc
Accession NumberP10696
ConstructionIle20-Asp503
Protein Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing 25 mM Tris, 100 mM NaCl, 1 mM MgCl2 (pH 7.5). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
ReconstitutionReconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
SynonymsPLAP-like, GCAP, ALPPL2, ALPPL, ALPG, ALP-1, Alkaline phosphatase, placental-like, Alkaline Phosphatase
Research Background
Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.
Chemical Properties
Molecular Weight79.33 kDa (predicted). Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result.
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

ALPPL-2ALPPL 2ALP1ALP 1
Related Tags: Alkaline Phosphatase (Germ type) /ALPG Protein, Human, Recombinant (hFc) chemical structure | Alkaline Phosphatase (Germ type) /ALPG Protein, Human, Recombinant (hFc) molecular weight