alpha Smooth Muscle Actin antibody
Référence GTX100034-100ul
Conditionnement : 100ul
Marque : Genetex
Host | Rabbit |
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Clonality | Polyclonal |
Isotype | IgG |
Application | WB, ICC/IF, IHC-P, IHC-Fr |
Reactivity | Human, Mouse, Rat, Zebrafish |
Package | 100 μl, 25 μl |
APPLICATION
Application Note
Application | Recommended Dilution |
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WB | 1:500-1:3000 |
ICC/IF | 1:100-1:1000 |
IHC-P | 1:100-1:1000 |
IHC-Fr | Assay dependent |
Calculated MW
Positive Control
Predict Reactivity
PROPERTIES
Form
Buffer
Preservative
Storage
Concentration
Antigen Species
Immunogen
Purification
Conjugation
RRID
Note
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Cellular Localization
Background
Database
DATA IMAGES
GTX100034 WB ImageNon-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
GTX100034 IHC-P Imagealpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse cervix.Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:1000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
GTX100034 IHC-P Imagealpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat cervix.Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:1000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
GTX100034 IHC-P Imagealpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis (Autostainer Formulated).Sample: Paraffin-embedded human normal colon tissue.alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:500.Antigen Retrieval: Citrate buffer, 20 min |
GTX100034 IHC-P Imagealpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat cervix.alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
GTX100034 IHC-P Imagealpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse prostate.Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:1000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
GTX100034 WB Imagealpha smooth muscle Actin antibody detects alpha smooth muscle Actin protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha smooth muscle Actin antibody (GTX100034) diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
GTX100034 WB Image alpha smooth muscle Actin antibody detects ACTA2 protein by western blot analysis. |
GTX100034 IHC-P Image alpha smooth muscle Actin antibody detects alpha smooth muscle Actin protein at cytoplasm on human liver carcinoma stroma by immunohistochemical analysis. |
GTX100034 IHC-P Imagealpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat stomach.alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
GTX100034 WB ImageWhole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
GTX100034 IHC-P Imagealpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat stomach.Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:1000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
GTX100034 IHC-P Imagealpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse duodenum.alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
GTX100034 IHC-P Imagealpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
GTX100034 IHC-P Imagealpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse duodenum.alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
GTX100034 IHC-P Imagealpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse smooth muscle.alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
GTX100034 WB ImageVarious tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
GTX100034 IHC-P Imagealpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse smooth muscle.alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
GTX100034 WB ImageVarious tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
GTX100034 IHC-P Imagealpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse smooth muscle.alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
GTX100034 WB ImageVarious whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
GTX100034 WB ImageVarious tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:200000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
GTX100034 WB ImageThe data was published in the 2022 in J Biomed Sci. PMID: 35765067 |
GTX100034 WB ImageThe data was published in the journal Biomolecules in 2019. PMID: 31443530 |
GTX100034 IHC-P ImageThe data was published in the journal PLoS One in 2019. PMID: 30695033 |
GTX100034 WB ImageThe data was published in the journal Front Pharmacol in 2016. PMID: 27199755 |
Application Reference
REVIEW
alpha Smooth Muscle Actin antibody Cat. No. GTX100034
Rating | | ( Average 4.4 based on 5 users reviews) |
Western Blot(WB) | | ( Average 4 based on 2 users reviews) |
Immunocytochemistry/ Immunofluorescence(ICC/IF) | | ( Average 4 based on 1 users reviews) |
IHC (Paraffin sections)(IHC-P) | | ( Average 5 based on 2 users reviews) |
SDS | |
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PBS.pdf | |
Glycerol.pdf | |
Proclin.pdf |
Document | |
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Unmodified.pdf |