Anti-ALKALINE PHOSPHATASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Alkaline Phosphatase Calf Intestine. Cross reactivity against Alkaline Phosphatase from other tissues and species may occur but have not been specifically determined.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration
lot specific
Conjugation
Unconjugated
Storage
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Stability
Expiration date is one (1) year from date of receipt.
In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney). Alkaline Phosphatase catalytic activity is phosphate monoester + H2O = an alcohol + phosphate.
Anti-Alkaline Phosphatase has been assayed against 1.0 ug of Alkaline Phosphatase Calf intestine in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG L - Cat# H (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-3-ethylbenthiazoline-6-sulfonic acid) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product. Physical State: Liquid (sterile filtered)
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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