Rat monoclonal antibody APC labled to CD107a

Description: Rat monoclonal antibody APC labled to CD107a Immunogen: This monoclonal antibody was raised against NIH/3T3 mouse embryo fobroblast tissue culture cell membranes. It has been mapped to the N-terminus of LAMP-1. Purification: The antibody was purified by affinity chromatography. Clonality: Monoclonal (clone: 1D4B) Conjugation: APC Form: Rat IgG2a kappa. Liquid in PBS, pH 7.3, 0.2% BSA, and 0.02% sodium azide. Dilution: 10 μl / assay Gene Symbol: LAMP1 Alternative Names: Lysosome-associated membrane glycoprotein 1; LAMP-1; Lysosome-associated membrane protein 1; CD107 antigen-like family member A; CD107aEntrez Gene (Mouse) : SwissProt (Mouse) : Directions for Use : 1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).Storage/Stability : Shipped and store at 4°C for one year. Do not freeze.