Armenian Hamster monoclonal antibody APC labled to CD11c

Référence CFB8776-100

Conditionnement : 100Assays

Marque : Cohesion Biosciences

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Product NameAnti-CD11c Antibody-APC labled
Cat No: CFB8776
Source: Armenian Hamster
Reactivity: M
Applications: IF, FC
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Description: Armenian Hamster monoclonal antibody APC labled to CD11c
Immunogen: Mouse spleen dendritic cells
Purification: The antibody was purified by affinity chromatography.
Clonality: Monoclonal (clone: N418)
Conjugation: APC
Form: Armenian Hamster IgG. Liquid in PBS, pH 7.3, 0.2% BSA, and 0.02% sodium azide.
Dilution: 10 μl / assay
Gene Symbol: ITGAX
Alternative Names: CD11C; Integrin alpha-X; CD11 antigen-like family member C; Leu M5; Leukocyte adhesion glycoprotein p150,95 alpha chain; Leukocyte adhesion receptor p150,95; CD11c
Entrez Gene (Mouse) :
SwissProt (Mouse) :
Directions for Use : 1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
Storage/Stability : Shipped and store at 4°C for one year. Do not freeze.