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> Anti-FGF5 Polyclonal Antibody

Anti-FGF5 Polyclonal Antibody

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Référence NB-64-155944-50uL

Conditionnement : 50uL

Marque : Neo Biotech


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Anti-FGF5 Polyclonal Antibody

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Catalog No. TMAB-05994 Copy Product Info
Anti-FGF5 Polyclonal Antibody is a Rabbit antibody targeting FGF5. Anti-FGF5 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Information

Bioactivity
Description
Anti-FGF5 Polyclonal Antibody is a Rabbit antibody targeting FGF5. Anti-FGF5 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Chicken,Dog,Cow)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by microwave in sodium citratebuffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3%BSA) at RTfor 30 min; Antibody incubation with (FGF5) Polyclonal/MonoclonalAntibody, Unconjugated (TMAB-05994) at 1:400 overnight at 4℃,followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by microwave in sodium citratebuffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3%BSA) at RTfor 30 min; Antibody incubation with (FGF5) Polyclonal/MonoclonalAntibody, Unconjugated (TMAB-05994) at 1:400 overnight at 4℃,followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (human placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (FGF5) Polyclonal Antibody, Unconjugated (TMAB-05994) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (human stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (FGF5) Polyclonal Antibody, Unconjugated (TMAB-05994) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
Application
IHC-P
IHC-Fr
IF
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationSecreted.
Tissue SpecificityExpressed in neonatal brain.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThe protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This gene was identified as an oncogene, which confers transforming potential when transfected into mammalian cells. Targeted disruption of the homolog of this gene in mouse resulted in the phenotype of abnormally long hair, which suggested a function as an inhibitor of hair elongation. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq].
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human FGF5
Antigen Species
Human
Gene Name
FGF5
Gene ID
2250
Protein Name
Fibroblast growth factor 5
Uniprot ID
P12034
Function
Functions as an inhibitor of hair elongation by promoting progression from anagen, the growth phase of the hair follicle, into catagen the apoptosis-induced regression phase.
Chemical Properties
Molecular WeightTheoretical: 29 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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