Rabbit polyclonal antibody to HSP90 alpha

Référence CPA1558-100ul

Conditionnement : 100ul

Marque : Cohesion Biosciences

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  • Western blot analysis of HSP90 alpha expression in HEK293T (A), Hela (B), A549 (C), mouse liver (D), mouse testis (E) whole cell lysates.
  • Immunohistochemical analysis of HSP90 alpha staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Immunofluorescent analysis of HSP90 alpha staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Product NameAnti-HSP90 alpha Antibody
Cat No: CPA1558
Source: Rabbit
Reactivity: H, M, R, B, C, D, Mk, P, Rb
Applications: WB, IH, IF/IC
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Description: Rabbit polyclonal antibody to HSP90 alpha
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human HSP90 alpha. The exact sequence is proprietary.
Purification: The antibody was purified by immunogen affinity chromatography.
Clonality: Polyclonal
Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
Gene Symbol: HSP90AA1
Alternative Names: HSP90A; HSPC1; HSPCA; Heat shock protein HSP 90-alpha; Heat shock 86 kDa; HSP 86; HSP86; Lipopolysaccharide-associated protein 2; LAP-2; LPS-associated protein 2; Renal carcinoma antigen NY-REN-38
Entrez Gene (Human):
Entrez Gene (Mouse) :
Entrez Gene (Rat) :
SwissProt (Human):
SwissProt (Mouse) :
SwissProt (Rat) :
Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.