Anti-Human IgM µ-Chain Specific (Clone HB57) - Biotin

Référence I-122-0.25

Conditionnement : 0.25mg

Marque : Leinco Technologies


AntiHuman IgM µChain Specific (Clone HB57) – Biotin

Product No.: I122

[product_table name="All Top" skus="I122"]

Clone
HB57
Target
Human Immunoglobulin
IgM µChain Specific
Formats AvailableView All
Product Type
Monoclonal Antibody
Isotype
IgG1
Applications
ELISA Det
,
FC

Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Product Concentration
0.5 mg/ml
Formulation
This biotinylated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This biotinylated antibody is stable when stored at 28°C. Do not freeze.
Cross Reactivity
Crossreactivity by ELISA against human myeloma proteins:
Human IgG1: 100%
Human IgG2: 100%
Human IgG3: 100%
Human IgG4: 100%
Human IgM: <0.2%
Human IgA: <0.1%
Human IgE: <0.1%

There is no detectable binding to bovine, goat, horse sheep IgG.

NOTE: This AntiHuman IgG, Fc Fragment Specific (Clone HB57) when conjugated to HRP is validated for use in Enzyme Immunoassay for the detection of Human IgG, µChain Specific (Part No.: I1201)
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
FC This antibody has been quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
AntiHuman IgM (µ chain specific) monoclonal antibody reacts with human IgM through an epitope on the heavy chain. This antibody is nonreactive with IgG, IgA or light chains. Clone HB57 produces an antibody with one of the highest affinities available (Ka = 5.34 x 108 M1).1
Antigen Distribution
Surface IgM is expressed on Blymphocytes.

References & Citations

1. Rudich, S. M. et al. (1988) J. Exp. Med. 168:247