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> Anti-MOUSE IgG2a (Texas Red™ Conjugated) Pre-adsorbed Secondary Antibody

Anti-MOUSE IgG2a (Texas Red™ Conjugated) Pre-adsorbed Secondary Antibody

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Référence ASR2732

Conditionnement : 1mg

Marque : Abcepta

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  • DB - Anti-MOUSE IgG2a (Texas Red™ Conjugated) Pre-adsorbed Secondary Antibody ASR2732
    Dot Blot of Goat anti-Mouse IgG2a Antibody Texas Red™ Conjugated Pre-absorbed. Antigen: Mouse IgG2a. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: n/a. Secondary antibody: Goat anti-Mouse IgG2a Antibody Texas Red™ Conjugated Pre-absorbed at 1:1,000 for 1 HR at RT. Block: MB-070 for 1 HR at RT.
Product Information
Description Anti-MOUSE IgG2a (Gamma 2a chain) (GOAT) Antibody Texas Red™ Conjugated (Min Cross Bv, Hu, and Rb Serum Proteins)
Host Goat
Conjugate Texas Red®
FP Value 2.1 moles Texas Red® per mole of IgG
Target Species Mouse
Reactivity Mouse
Clonality Polyclonal
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, IC
Application Note FLISA 1:10,000-1:50,000
IF Microscopy 1:1,000-1:5,000
Western Blot 1:4,000-1:20,000
Immunochemistry 1:1,000-1:5,000
Physical State Lyophilized
Host Isotype IgG
Target Isotype IgG2a
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Mouse IgG2a heavy chain
Reconstitution Volume 1.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Ambient
Purity MOUSE IgG2a Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG2a. Specificity was confirmed by ELISA at less than 1% cross-reactivity against other mouse heavy or light chain isotypes. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.
Storage Condition Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.