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> Anti-Phospho-GATA2 (Ser401) Polyclonal Antibody 2

Anti-Phospho-GATA2 (Ser401) Polyclonal Antibody 2

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Référence NB-64-160661-50uL

Conditionnement : 50uL

Marque : Neo Biotech


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Anti-Phospho-GATA2 (Ser401) Polyclonal Antibody 2

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Catalog No. TMAB-10729 Copy Product Info
Anti-Phospho-GATA2 (Ser401) Polyclonal Antibody 2 is a Rabbit antibody targeting Phospho-GATA2 (Ser401). Anti-Phospho-GATA2 (Ser401) Polyclonal Antibody 2 can be used in WB.
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Information

Bioactivity
Description
Anti-Phospho-GATA2 (Ser401) Polyclonal Antibody 2 is a Rabbit antibody targeting Phospho-GATA2 (Ser401). Anti-Phospho-GATA2 (Ser401) Polyclonal Antibody 2 can be used in WB.
Ig Type
IgG
Reactivity
Human (predicted:Pig,Horse,Rabbit)
Verified Activity
Sample: A549 Cell (Human) Lysate at 30 μg
Primary: Anti-p-GATA2 (Ser401) (TMAB-10729) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD
Application
WB
Recommended Dose
WB: 1:500-2000
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus.
Tissue SpecificityEndothelial cells.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundMembers of the GATA family share a conserved zinc finger DNA-binding domain and are capable of binding the WGATAR consensus sequence. GATA-1 is erythroid-specific and is responsible for the regulated transcription of erythroid genes. It is an essential component in the generation of the erythroid lineage. GATA-2 is expressed in embryonic brain and liver, HeLa and endothelial cells, as well as in erythroid cells. Studies with a modified GATA consensus sequence, AGATCTTA, have shown that GATA-2 and GATA-3 recognize this mutated consensus while GATA-1 has poor recognition of this sequence. This indicates broader regulatory capabilities of GATA-2 and GATA-3 than GATA-1. GATA-3 is highly expressed in T lymphocytes. GATA-4, GATA-5 and GATA-6 comprise a subfamily of transcription factors. Both GATA-4 and GATA-6 are found in heart, pancreas and ovary; lung and liver tissues exhibit GATA-6, but not GATA-4 expression. GATA-5 expression has been observed in differentiated heart and gut tissues and is present throughout the course of development in the heart. Although expression patterns of the various GATA transcription factors may overlap, it is not yet apparent how the GATA factors are able to discriminate in binding their appropriate target sites.
Antigen Details
Immunogen
KLH conjugated synthesised phosphopeptide: human GATA2 around the phosphorylation site of Ser401
Antigen Species
Human
Gene Name
GATA2
Gene ID
2624
Protein Name
Endothelial transcription factor GATA-2
Uniprot ID
P23769
Function
Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'.
Chemical Properties
Molecular WeightTheoretical: 53 kDa. Actual: 53 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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