Anti-Phospho-TGF beta Receptor II (Ser225) Polyclonal Antibody
Référence NB-64-161302-100uL
Conditionnement : 100uL
Marque : Neo Biotech
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Anti-Phospho-TGF beta Receptor II (Ser225) Polyclonal Antibody
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Catalog No. TMAB-11374 Copy Product Info
Anti-Phospho-TGF beta Receptor II (Ser225) Polyclonal Antibody is a Rabbit antibody targeting Phospho-TGF beta Receptor II (Ser225). Anti-Phospho-TGF beta Receptor II (Ser225) Polyclonal Antibody can be used in WB.
For research use only—not for human use. No sales to individuals. Use as intended only.
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| Description | Anti-Phospho-TGF beta Receptor II (Ser225) Polyclonal Antibody is a Rabbit antibody targeting Phospho-TGF beta Receptor II (Ser225). Anti-Phospho-TGF beta Receptor II (Ser225) Polyclonal Antibody can be used in WB. |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Dog,Pig,Rabbit,Sheep) |
| Verified Activity | 1. Sample: Lumph node (Mouse) Lysate at 40 μg Primary: Anti-p-TGF beta Receptor Ii (Ser225) (TMAB-11374) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62 kD Observed band size: 64 kD 2. Sample: Lung (Mouse) Lysate at 40 μg Primary: Anti-p-TGF beta Receptor Ii (Ser225) (TMAB-11374) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62 kD Observed band size: 64 kD 3. Sample: Spleen (Mouse) Lysate at 40 μg Primary: Anti-p-TGF beta Receptor Ii (Ser225) (TMAB-11374) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62 kD Observed band size: 64 kD |
| Application | WB |
| Recommended Dose | WB: 1:500-2000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane; Single-pass type I membrane protein. |
| Tissue Specificity | Phosphorylated on a Ser/Thr residue in the cytoplasmic domain. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| Concentration | 1mg/ml |
| Research Background | This gene encodes a member of the Ser/Thr protein kinase family and the TGFB receptor subfamily. The encoded protein is a transmembrane protein that has a protein kinase domain, forms a heterodimeric complex with another receptor protein, and binds TGF-beta. This receptor/ligand complex phosphorylates proteins, which then enter the nucleus and regulate the transcription of a subset of genes related to cell proliferation. Mutations in this gene have been associated with Marfan Syndrome, Loeys-Deitz Aortic Aneurysm Syndrome, and the development of various types of tumors. Alternatively spliced transcript variants encoding different isoforms have been characterized. |
| Immunogen | KLH conjugated synthesised phosphopeptide: human TGF beta Receptor II around the phosphorylation site of Ser225 |
| Antigen Species | Human |
| Gene Name | Tgfbr2 |
| Gene ID | 7048 |
| Protein Name | TGF-beta receptor type-2 |
| Uniprot ID | P37173 |
| Function | Transmembrane serine/threonine kinase forming with the TGF-beta type I serine/threonine kinase receptor, TGFBR1, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and the activation of TGFRB1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade. Also involved in non-canonical, SMAD-independent TGF-beta signaling pathways. |
| Molecular Weight | Theoretical: 62 kDa. Actual: 62 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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