Anti-Porcine TNF-α (Clone 103304)

E. coliDerived Recombinant Porcine TNFα

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

The lyophilized antibody can be stored desiccated at 20°C to 70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 28°C. For longterm storage of the reconstituted antibody, aseptically aliquot into working volumes and store at 20°C to 70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

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Clone 103304 recognizes an epitope on mouse TNFα.

TNFα is a 17.5 kD protein that mediates inflammation and immunity caused by the invasion of viruses, bacteria, and parasites by initiating a cascade of cytokines that increase vascular permeability, thus bringing macrophages and neutrophils to the site of infection. TNFα secreted by the macrophage causes the blood to clot which provides containment of the infection. TNFα binding to surface receptors brings about various biologic activities that include cytolysis and cytostasis of many tumor cell lines In vitro, hemorraghic necrosis of tumors In vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.

TNFα is secreted by macrophages, monocytes, neutrophils, T cells, B cells, NK cells, LAK cells.

1. ElHarith, EHA. et al. (2004) Saudi Med J. 25: 135
2. Adolf, GR. et al. (1990) Infec Immun. 58: 3996