Anti-SETD2 (SET Domain-containing Protein 2, Histone-lysine N-methyltransferase SETD2, HYPB, SET2, HIF-1, HIP-1, KMT3A, HBP231, HSPC069, p231HBP) Polyclonal Antibody

Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. This gene encodes a protein belonging to a class of huntingtin interacting proteins characterized by WW motifs. This protein is a histone methyltransferase that is specific for lysine-36 of histone H3, and methylation of this residue is associated with active chromatin. This protein also contains a novel transcriptional activation domain and has been found associated with hyperphosphorylated RNA polymerase II.||Applications: |Suitable for use in Immunofluorescence/Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, ELISA and Western Blot. Other applications not tested.||Recommended Dilutions:|Western Blot: 1:500-1:1000|Immunohistochemistry (Paraffin) : 1:500-1:1000. High pressure antigen retrieval performed with citrate buffer, pH 6.0 is recommended.|Immunofluorescence (IC): 1:50-1:200|Immunoprecipitation: 0.5ug-4ug for 400ug-600ug extracts of whole cells|Optimal dilutions to be determined by the researcher.||Storage and Stability: |May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.