Arginase 1 antibody
Référence GTX109242-100ul
Conditionnement : 100ul
Marque : Genetex
Contactez votre distributeur local :
Téléphone : +1 850 650 7790
9
Host | Rabbit |
|---|---|
Clonality | Polyclonal |
Isotype | IgG |
Application | WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA, Sandwich ELISA |
Reactivity | Human, Mouse, Rat |
| Package | 100 μl, 25 μl |
Datasheet File View product citations for antibody GTX109242 on CiteAb
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application | Recommended Dilution |
|---|---|
| WB | 1:500-1:3000 |
| ICC/IF | 1:100-1:1000 |
| IHC-P | 1:100-1:1000 |
| IHC-Fr | 1:100-1:1000 |
| FACS | 1:50-1:200 |
| IP | 1:100-1:1000 |
| ELISA | 1:1000-1:10000 |
| Sandwich ELISA | Assay dependent |
| Note : Sandwich ELISACapture : GTX634218 / GTX109242, Detection : GTX109242 / GTX634218 | |
Not tested in other applications.
Calculated MW
35 kDa. ( Note )
Positive Control
HepG2
Predict Reactivity
Rabbit, Bovine, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.26 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Full length human Arginase 1 Recombinant protein.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2036264
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
arginase 1
Cellular Localization
Cytoplasm
Background
Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. [provided by RefSeq]
Database
DATA IMAGES
| | GTX109242 WB ImageNon-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody (GTX109242) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
| | GTX109242 WB ImageNon-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody (GTX109242) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
| | GTX109242 ELISA ImageSandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634218 as capture antibody at concentration of 5 μg/mL and GTX109242 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody. |
| | GTX109242 IHC-P ImageArginase 1 antibody detects Arginase 1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse liver.Arginase 1 stained by Arginase 1 antibody (GTX109242) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
| | GTX109242 IP Image Immunoprecipitation of Arginase 1 protein from HepG2 whole cell extracts using 5 μg of Arginase 1 antibody (GTX109242). |
| | GTX109242 IHC-Fr ImageArginase 1 antibody detects Arginase 1 protein by immunohistochemical analysis.Sample: Frozen-sectioned mouse hippocampus.Green: Arginase 1 stained by Arginase 1 antibody (GTX109242) diluted at 1:250.Blue: Fluoroshield with DAPI (GTX30920). |
| | GTX109242 WB ImageVarious tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody (GTX109242) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
| | GTX109242 FACS Image Arginase 1 antibody (GTX109242) detects ARG1 protein by flow cytometry analysis. |
| | GTX109242 ELISA ImageSandwich ELISA detection of recombinant full-length Arginase 1 protein using GTX109242 as capture antibody at concentration of 5 μg/mL and GTX634218 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody. |
| | GTX109242 ICC/IF ImageArginase 1 antibody detects Arginase 1 protein at cytoplasm by immunofluorescent analysis.Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Arginase 1 stained by Arginase 1 antibody (GTX109242) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).Scale bar= 10 μm. |
| | GTX109242 WB ImageVarious whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody (GTX109242) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program. |
Application Reference
Trujillo MN et al. Mol Metab 2024; 81 : 101888 Lactoylglutathione promotes inflammatory signaling in macrophages through histone lactoylation.
Bokil AA et al. Cancer Cell Int 2023; 23 (1) : 279 Discovery of a new marker to identify myeloid cells associated with metastatic breast tumours.
Mossmann D et al. Cell 2023; 186 (23) : 5068-5083.e23 Arginine reprograms metabolism in liver cancer via RBM39.
Shosha E et al. Cell Death Dis 2023; 14 (9) : 621 The arginase 1/ornithine decarboxylase pathway suppresses HDAC3 to ameliorate the myeloid cell inflammatory response: implications for retinal ischemic injury.
Schaafsma E et al. Cancer Immunol Res 2023; 11 (1) : 38-55 VISTA Targeting of T-cell Quiescence and Myeloid Suppression Overcomes Adaptive Resistance.
Submit a Reference REVIEW
Arginase 1 antibody Cat. No. GTX109242
| Rating | | ( Average 4.8 based on 9 users reviews) |
| Western Blot(WB) | | ( Average 4.8 based on 8 users reviews) |
| IHC (Paraffin sections)(IHC-P) | | ( Average 5 based on 1 users reviews) |
Application Reference
Trujillo MN et al. Mol Metab 2024; 81:101888 Lactoylglutathione promotes inflammatory signaling in macrophages through histone lactoylation.
Bokil AA et al. Cancer Cell Int 2023; 23 (1):279 Discovery of a new marker to identify myeloid cells associated with metastatic breast tumours.
Mossmann D et al. Cell 2023; 186 (23):5068-5083.e23 Arginine reprograms metabolism in liver cancer via RBM39.
Shosha E et al. Cell Death Dis 2023; 14 (9):621 The arginase 1/ornithine decarboxylase pathway suppresses HDAC3 to ameliorate the myeloid cell inflammatory response: implications for retinal ischemic injury.
Schaafsma E et al. Cancer Immunol Res 2023; 11 (1):38-55 VISTA Targeting of T-cell Quiescence and Myeloid Suppression Overcomes Adaptive Resistance.
Yang JX et al. Neuroreport 2023; 34 (12):638-648 Electroacupuncture relieves chronic pain by promoting microglia M2 polarization in lumbar disc herniation rats.
Panebianco C et al. Cell Death Discov 2023; 9 (1):116
Application : IHC-P
Reactivity : Mouse
Huang J et al. Mol Metab 2023; 67:101651 Scavenging dicarbonyls with 5'-O-pentyl-pyridoxamine increases HDL net cholesterol efflux capacity and attenuates atherosclerosis and insulin resistance.
Hofmann L et al. Cancers (Basel) 2023; 15 (22) Arginase-1 in Plasma-Derived Exosomes as Marker of Metastasis in Patients with Head and Neck Squamous Cell Carcinoma.
Anderson S et al. Biomedicines 2022; 10 (8) Dental Pulp-Derived Stem Cells Reduce Inflammation, Accelerate Wound Healing and Mediate M2 Polarization of Myeloid Cells.
Application : WB
Reactivity : Mouse
Broome ST et al. J Mol Neurosci 2022; 72 (11):2163-2175 PACAP and VIP Mitigate Rotenone-Induced Inflammation in BV-2 Microglial Cells.
Jian Wang et al. Theranostics 2022; 12 (9):4288-4309 Grafted human ESC-derived astroglia repair spinal cord injury via activation of host anti-inflammatory microglia in the lesion area.
Application : WB, IHC-Fr
Reactivity : Mouse
Le Liu et al. Front Pediatr 2022; 10:867410 Multi-Hit White Matter Injury-Induced Cerebral Palsy Model Established by Perinatal Lipopolysaccharide Injection.
Application : WB
Reactivity : Mouse
Ashenafi S et al. Am J Pathol 2022; (Epub) Immunosuppressive Features of the Microenvironment in Lymph Nodes Granulomas from Tuberculosis and HIV-Co-Infected Patients.
Application : IHC-P
Reactivity : Human
Wu L et al. Theranostics 2022; 12 (2):842-858 FcγRIIB potentiates differentiation of myeloid-derived suppressor cells to mediate tumor immunoescape.
Reactivity : Mouse
Wilson AJ et al. Neoplasia 2022; 24 (2):63-75 Panobinostat enhances olaparib efficacy by modifying expression of homologous recombination repair and immune transcripts in ovarian cancer.
Application : IHC-P
Reactivity : Human
Libu?e Jansk? et al. Dis Model Mech 2021; 14 (8) The MEMIC is an ex vivo system to model the complexity of the tumor microenvironment.
Application : ICC/IF
Reactivity : Mouse
Wang K et al. Transl Neurosci 2021; 12 (1):469-481 Cryptotanshinone ameliorates CUS-induced depressive-like behaviors in mice.
Application : WB
Reactivity : Mouse
Grzywa TM et al. Commun Biol 2021; 4 (1):1384 Potent but transient immunosuppression of T-cells is a general feature of CD71+ erythroid cells.
Application : WB
Reactivity : Human
Cai M et al. CNS Neurosci Ther 2021; 27 (11):1348-1365 Sevoflurane preconditioning protects experimental ischemic stroke by enhancing anti-inflammatory microglia/macrophages phenotype polarization through GSK-3β/Nrf2 pathway.
Application : ICC/IF
Vilhena ER et al. Behav Pharmacol 2021; 32 (6):459-471 Positive effects of roflumilast on behavior, neuroinflammation, and white matter injury in mice with global cerebral ischemia.
Dichtl S et al. Sci Adv 2021; 7 (26) Lactate and IL6 define separable paths of inflammatory metabolic adaptation.
Application : WB
Reactivity : Mouse
Tzeng HT et al. Cancers (Basel) 2021; 13 (6) Plasminogen Activator Inhibitor-1 Secretion by Autophagy Contributes to Melanoma Resistance to Chemotherapy through Tumor Microenvironment Modulation.
Application : IHC-P
Reactivity : Mouse
Sturgill ER et al. Oncoimmunology 2021; 10 (1):1892265 Galectin-3 inhibition with belapectin combined with anti-OX40 therapy reprograms the tumor microenvironment to favor anti-tumor immunity.
Application : IHC-P
Reactivity : Mouse
Tingting Wang et al. Front Cell Dev Biol 2020; 8:593890 DJ-1 Regulates Microglial Polarization Through P62-Mediated TRAF6/IRF5 Signaling in Cerebral Ischemia-Reperfusion.
Application : WB
Reactivity : Rat
Hoover AA et al. BMC Cancer 2020; 20 (1):970 Increased canonical NF-kappaB signaling specifically in macrophages is sufficient to limit tumor progression in syngeneic murine models of ovarian cancer.
Scherwitzl I et al. Mol Ther Oncolytics 2020; 17:431-447 Sindbis Virus with Anti-OX40 Overcomes the Immunosuppressive Tumor Microenvironment of Low-Immunogenic Tumors.
Nagumo Y et al. Microsurgery 2020; 40 (8):886-895 Use of sliced or minced peripheral nerve segments for nerve regeneration through a biodegradable nerve conduit: A preliminary study in the rat.
Application : IHC-P
Reactivity : Rat
Lee YL et al. J Food Biochem 2020;:e13469 Extract of Pyrus nivalis enhances phagocytosis in lungs after particles matter exposure in BALB/c mice.
Application : IHC-P
Reactivity : Mouse
Hanna A et al. Am J Physiol Heart Circ Physiol 2020; 319 (5):H948-H964 Validation of diagnostic criteria and histopathological characterization of cardiac rupture in the mouse model of nonreperfused myocardial infarction.
Application : ICC/IF
Reactivity : Mouse
Principe M et al. J Clin Endocrinol Metab 2020; 105 (11) Immune Landscape of Pituitary Tumors Reveals Association Between Macrophages and Gonadotroph Tumor Invasion.
Application : ICC/IF, IHC-P
Reactivity : Mouse
Sui A et al. Invest Ophthalmol Vis Sci 2020; 61 (6):4 Inhibiting NF-κB Signaling Activation Reduces Retinal Neovascularization by Promoting a Polarization Shift in Macrophages.
Application : WB
Reactivity : Mouse
Wang W et al. Acta Biomater 2020; 108:223-236 Advances in the Masquelet technique: Myeloid-derived suppressor cells promote angiogenesis in PMMA-induced membranes.
Application : FACS
Reactivity : Mouse
Arturo A Wilkins-Rodr?guez et al. Infect Immun 2020; 88 (7) Differential Regulation of l-Arginine Metabolism through Arginase 1 during Infection with Leishmania mexicana Isolates Obtained from Patients with Localized and Diffuse Cutaneous Leishmaniasis.
Application : WB
Taisuke Okano et al. International Journal of Endocrinology 2020; 2020:Article ID 4139093 β-Endorphin Mediates the Development and Instability of Atherosclerotic Plaques
Application : WB
Reactivity : Human
Di Domenico F et al. Redox Biol 2019; 23:101162 Restoration of aberrant mTOR signaling by intranasal rapamycin reduces oxidative damage: Focus on HNE-modified proteins in a mouse model of down syndrome.
Zhang D et al. Nature 2019; 574 (7779):575-580 Metabolic regulation of gene expression by histone lactylation.
Glass EB et al. ACS Omega 2019; 4 (16):16756-16767 Optimizing Mannose "Click" Conjugation to Polymeric Nanoparticles for Targeted siRNA Delivery to Human and Murine Macrophages.
Application : WB
Reactivity : Mouse
He Y et al. Int Immunopharmacol 2019; 77:105929 Ethyl pyruvate enhances spontaneous remyelination by targeting microglia phagocytosis.
Application : IHC-P
Reactivity : Mouse
Tateyama H et al. Immunology 2019; Siglec-F is induced by granulocyte-macrophage colony-stimulating factor and enhances interleukin-4-induced expression of arginase-1 in mouse macrophages.
Matsui S et al. Am J Pathol 2019; Endogenous calcitonin gene-related peptide deficiency exacerbates postoperative lymphedema by suppressing lymphatic capillary formation and M2 macrophage accumulation.
Application : IHC-P
Reactivity : Mouse
Barati S et al. J Cell Biochem 2019; (Epub) Mesenchymal stem cell mediated effects on microglial phenotype in cuprizone-induced demyelination model.
Application : IHC-P
Reactivity : Mouse
Koizumi T et al. J Neuroinflammation 2019; 16 (1):79 Transiently proliferating perivascular microglia harbor M1 type and precede cerebrovascular changes in a chronic hypertension model.
Application : WB
Reactivity : Rat
Tziakas DN et al. Circulation 2019; (Epub) Lysed Erythrocyte Membranes Promote Vascular Calcification: Possible Role of Erythrocyte-Derived Nitric Oxide.
Application : FACS
Reactivity : Human
Motterlini R et al. Redox Biol 2019; 20:334-348 HYCO-3, a dual CO-releaser/Nrf2 activator, reduces tissue inflammation in mice challenged with lipopolysaccharide.
Application : IHC-P
Reactivity : Mouse
Gualdr籀n-L籀pez M et al. Front Microbiol 2018; 9:1271 Characterization of Plasmodium vivax Proteins in Plasma-Derived Exosomes From Malaria-Infected Liver-Chimeric Humanized Mice.
Pearse DD et al. Int J Mol Sci 2018; 19 (9) Schwann Cell Transplantation Subdues the Pro-Inflammatory Innate Immune Cell Response after Spinal Cord Injury.
Polis B et al. Neurotherapeutics 2018; 15 (4):1036-1054 L-Norvaline Reverses Cognitive Decline and Synaptic Loss in a Murine Model of Alzheimer's Disease.
Application : IHC-P, IHC-Fr
Reactivity : Mouse
Gomez D et al. Nat Med 2018; (Epub) Interleukin-1β has atheroprotective effects in advanced atherosclerotic lesions of mice.
Application : IHC-P
Reactivity : Mouse
Yan G et al. Cancer Res 2018; (Epub) A RIPK3-PGE2 circuit mediates myeloid-derived suppressor cell-potentiated colorectal carcinogenesis.
Application : FACS
Reactivity : Mouse
Jang TJ et al. Pathol Res Pract 2018; (Epub) Increased number of arginase 1-positive cells in the stroma of carcinomas compared to precursor lesions and nonneoplastic tissues.
Application : IHC-P
Reactivity : Human
Carmona-Fontaine C et al. Proc Natl Acad Sci U S A 2017; 114 (11):2934-2939 Metabolic origins of spatial organization in the tumor microenvironment.
Shen P et al. BMC Microbiol 2017; 17 (1):185 IRAK-M alters the polarity of macrophages to facilitate the survival of Mycobacterium tuberculosis.
Application : WB
Reactivity : Human
Zhu Y et al. Int J Mol Med 2017; (Epub) Identification of different macrophage subpopulations with distinct activities in a mouse model of oxygen-induced retinopathy.
Pileri A et al. Virchows Arch 2017; (Epub) Langerhans, plasmacytoid dendritic and myeloid-derived suppressor cell levels in mycosis fungoides vary according to the stage of the disease.
Application : IHC-P
Reactivity : Human
Wu J et al. J Neuroinflammation 2017; 14 (1):36 Roles of programmed death protein 1/programmed death-ligand 1 in secondary brain injury after intracerebral hemorrhage in rats: selective modulation of microglia polarization to anti-inflammatory phenotype.
Application : WB
Reactivity : Rat
Royo F et al. Sci Rep 2017; 7:42798 Hepatocyte-secreted extracellular vesicles modify blood metabolome and endothelial function by an arginase-dependent mechanism.
Application : WB
Reactivity : Rat
Romano A et al. Oncotarget 2016; 7 (41):67333-67346 The prognostic value of the myeloid-mediated immunosuppression marker Arginase-1 in classic Hodgkin lymphoma.
Application : IHC-P
Reactivity : Human
Aalinkeel R et al. J Neuroimmune Pharmacol 2016; (Epub) Galectin-1 Reduces Neuroinflammation via Modulation of Nitric Oxide-Arginase Signaling in HIV-1 Transfected Microglia: a Gold Nanoparticle-Galectin-1 "Nanoplex" a Possible Neurotherapeutic?
Application : ICC/IF
Reactivity : Human
Ghosh M et al. J Neuroinflammation 2016; 13:9 Cyclic AMP is a key regulator of * to M2a phenotypic conversion of microglia in the presence of Th2 cytokines.
Application : WB, ICC/IF
Reactivity : Mouse
Le Noci V et al. Oncoimmunology 2015; 4 (10):e1040214 Poly(I:C) and CpG-ODN combined aerosolization to treat lung metastases and counter the immunosuppressive microenvironment.
Application : IHC-P
Reactivity : Mouse
Mei S et al. PLoS One 2015; 10 (8):e0135867 MicroRNA-200c Promotes Suppressive Potential of Myeloid-Derived Suppressor Cells by Modulating PTEN and FOG2 Expression.
Application : WB
Reactivity : Mouse
Jindal A et al. Exp Mol Pathol 2015; 99 (1):155-62 Fat-laden macrophages modulate lobular inflammation in nonalcoholic steatohepatitis (NASH).
Application : FACS
Reactivity : Mouse
Beachley VZ et al. Nat Methods 2015; (Epub) Tissue matrix arrays for high-throughput screening and systems analysis of cell function.
Application : ICC/IF
Reactivity : Mouse
Tavori H et al. J Lipid Res 2014; 55 (10):2073-81 Macrophage-derived apoESendai suppresses atherosclerosis while causing lipoprotein glomerulopathy in hyperlipidemic mice.
Reactivity : Mouse
Harmon EY et al. J Am Heart Assoc 2014; 3 (6):e001232 Anti-inflammatory immune skewing is atheroprotective: Apoe-/-FcγRIIb-/- mice develop fibrous carotid plaques.
Gladine C et al. Genes Nutr 2014; 9 (5):424 The omega-3 fatty acid docosahexaenoic acid favorably modulates the inflammatory pathways and macrophage polarization within aorta of LDLR(-/-) mice.
Application : IHC-Fr
Reactivity : Mouse
Yoshii A et al. Biol Reprod 2014; Role of Uterine Contraction in Regeneration of the Murine Postpartum Endometrium.
Application : IHC-Fr
Reactivity : Mouse
Sward K et al. PLoS One 2014; 9 (3):e92428 Arterial dysfunction but maintained systemic blood pressure in cavin-1-deficient mice.
Application : WB
Reactivity : Mouse
Alabanza LM et al. J Immunol 2013; 191 (7):3764-77 Inhibition of endogenous activated protein C attenuates experimental autoimmune encephalomyelitis by inducing myeloid-derived suppressor cells.
Application : ICC/IF
Reactivity : Mouse
Sward K et al. Physiol Rep 2013; 1 (1):e00008 Elevated pulmonary arterial pressure and altered expression of Ddah1 and Arg1 in mice lacking cavin-1/PTRF.
Guo Z et al. J Immunol 2013; 190 (8):4337-48 Antisense oligonucleotide treatment enhances the recovery of acute lung injury through IL-10-secreting M2-like macrophage-induced expansion of CD4+ regulatory T cells.
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