Anti-ATPase AAA2 domain
Référence AS111754
Conditionnement : 200ul
Marque : Agrisera
Contactez votre distributeur local :
Téléphone : +1 850 650 7790
Visar bild 1 av
1
Product no: AS11 1754
AS11 1754 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, C.reinhardtii, L. angustifolius, P. sativum, S. tuberosum, Synechococcus sp. PCC 7942, Z. mays L.
- Product Info
-
Immunogen: KLH-conjugated synthetic peptide derived from proteins containing AAA2 domain, including Arabidopsis thaliana ClpB1 P42730, At1g74310
Host: Rabbit Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 200 µl Reconstitution: For reconstitution add 200 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 2000 (WB) - Reactivity
-
Confirmed reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii, Lupinus angustifolius, Pisum sativum, Solanum tuberosum, Synechococcus sp. PCC 7942, Zea mays L. Predicted reactivity: AAA2 domain containing proteins including Saccharomyces cerevisiae HSP104. Nannochloropsis gaditana ClpB chaperone Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
-
Application example
The 20 μg of soluble proteins from 3-week old Solanum tuberosum (1) and Arabidopsis thaliana (2), one-week old Lupinus angustifolius (3), Pisum sativum (4) and Zea mays L. (5) leaves extracted with buffer containing 50 мM Hepes-KOH, pH 7.5, 330 мM sorbitol, 2 мM EDTA, 1 мM MgCl2, 5 мM ascorbate, 0.05% BSA were mixed with sample buffer and denatured for 5 min at 70°C. Samples were separated on 10% SDS-PAGE and blotted 1h to nitrocellulose membrane (Amersham Protran) using tank wet-transfer (Bio-Rad) in standard transfer buffer in presence of 20% methanol. Transfer of proteins to the membrane was checked using 1% Ponceau S staining before the blocking step. Blots were blocked in buffer (5% low-fat milk in 1xPBS, 0.1% Tween-20) for 1h at room temperature (RT) with agitation. Blots were incubated in the primary antibody at a dilution of 1:2 000 for 1 h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, and then washed once for 15 min and 3 times for 5 min in PBS-T at RT with agitation. Blot was incubated in secondary antibody (goat anti-rabbit IgG,Vous serez peut-être également intéressé par les produits suivants :
RéférenceDescriptionCond.Prix HT