BCIP [6578-06-9]
Référence HY-15909-100mg
Conditionnement : 100mg
Marque : MedChemExpress
BCIP(BCIP p-toluidine salt; X-phosphate p-toluidine salt) is an artificial chromogenic substrate used for the sensitive colorimetric detection of alkaline phosphatase activity.
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BCIP Chemical Structure
CAS No. : 6578-06-9
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Description |
BCIP(BCIP p-toluidine salt; X-phosphate p-toluidine salt) is an artificial chromogenic substrate used for the sensitive colorimetric detection of alkaline phosphatase activity. |
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Masse moléculaire |
433.62 |
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Formule |
C15H15BrClN2O4P |
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CAS No. |
6578-06-9 |
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Appearance |
Solid |
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Color |
White to off-white |
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SMILES |
ClC1=C(Br)C=CC2=C1C(OP(O)(O)=O)=CN2.NC3=CC=C(C)C=C3 |
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Livraison | Room temperature in continental US; may vary elsewhere. |
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Stockage |
4°C, sealed storage, away from moisture and light *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light) |
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Solvant et solubilité |
In Vitro:
DMSO : ≥ 51 mg/mL (117.61 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) DMF : 25 mg/mL (57.65 mM; Need ultrasonic) *"≥" means soluble, but saturation unknown. Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration:
mg/mL
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Pureté et documentation |
Purity: 99.36% |
Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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DMF / DMSO | 1 mM | 2.3062 mL | 11.5308 mL | 23.0617 mL | 57.6542 mL |
5 mM | 0.4612 mL | 2.3062 mL | 4.6123 mL | 11.5308 mL | |
10 mM | 0.2306 mL | 1.1531 mL | 2.3062 mL | 5.7654 mL | |
15 mM | 0.1537 mL | 0.7687 mL | 1.5374 mL | 3.8436 mL | |
20 mM | 0.1153 mL | 0.5765 mL | 1.1531 mL | 2.8827 mL | |
25 mM | 0.0922 mL | 0.4612 mL | 0.9225 mL | 2.3062 mL | |
30 mM | 0.0769 mL | 0.3844 mL | 0.7687 mL | 1.9218 mL | |
40 mM | 0.0577 mL | 0.2883 mL | 0.5765 mL | 1.4414 mL | |
50 mM | 0.0461 mL | 0.2306 mL | 0.4612 mL | 1.1531 mL | |
DMSO | 60 mM | 0.0384 mL | 0.1922 mL | 0.3844 mL | 0.9609 mL |
80 mM | 0.0288 mL | 0.1441 mL | 0.2883 mL | 0.7207 mL | |
100 mM | 0.0231 mL | 0.1153 mL | 0.2306 mL | 0.5765 mL |
BCIP Related Classifications
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Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.
Keywords:
BCIP6578-06-9X-phosphateFluorescent DyeInhibitorinhibitorinhibit