A8012-10mg | Biotin-XX Tyramide Reagent Clinisciences

Biotin-LC-LC-tyramide is used for tyramide signal amplification (TSA) which is a powerful, patented technology that significantly enhances both chromogenic and fluorescent signals. TSA is easily integrated into standard nonradioactive in situ hybridization (ISH) or IHC protocols.

TSA is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to catalyze the deposition of a fluorophore-labeled tyramide amplification reagent onto tissue sections or cell preparation surfaces that have been previously blocked with proteins.

Biotin-LC-LC-tyramide use horseradish peroxidase (HRP) to catalyze covalent deposition of biotin labels directly adjacent to the immobilized enzyme. The labeling reaction is quick (less than 10 minutes) and deposited labels can be detected with streptavidin conjugates for imaging in brightfield or fluorescence microscopy.

Physical Appearance	A solid
Storage	Store at -20°C
M.Wt	589.79
Formula	C₃₀H₄₇N₅O₅S
Synonyms	biotin-LC-LC-tyramide
Solubility	≥59 mg/mL in DMSO; insoluble in H2O; ≥14.1 mg/mL in EtOH with ultrasonic
Chemical Name	N-(4-hydroxyphenethyl)-6-(6-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanamido)hexanamide
SDF	Download SDF
Canonical SMILES	O=C1N[C@@](13)([C@](13)(CS2)N1)[C@@H]2CCCCC(NCCCCCC(NCCCCCC(NCCC3=CC=C(O)C=C3)=O)=O)=O
Shipping Condition	Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips	We do not recommend long-term storage for the solution, please use it up soon.