Specifications

Product Data
Clone Name	BRG-01
Applications	IHC, WB
Recommended Dilution	Western blot: 10 μg/ml Positive control: HEK293 cells. This clone has been described to work in Immunohistochemistry on Paraffin Sections.
Reactivities	Human
Host	Mouse
Isotype	IgG2a
Clonality	Monoclonal
Immunogen	Bacterially produced GST-fused protein representing 242 C-terminal amino acids of human Brg1
Specificity	The antibody BRG-01 recognizes an epitope within C-terminal part of Human Brg1, a 205 kDa catalytic subunit of SWI2/SNF2-family chromatin-remodeling complexes.
Formulation	PBS, pH~7.4 State: Aff - Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE) Preservative: 15 mM Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Gene Name	SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
Database Link	Entrez Gene 6597 Human UniProt ID P51532
Background	Brg1 (Brahma-related gene 1) is an ATPase subunit of SWI2/SNF2-like chromatin-remodeling complexes that enable access of regulatory and effector proteins in transcription, DNA repair and DNA replication. Although Brg1-containing complexes are not essential for general cell survival, they participate in transcriptional regulation of several hundred genes including those involved in interferon and stress response, immune cells differentiation, neurogenesis, cell cycle etc. and is absolutely necessary for mouse embryogenesis. Brg1 is also involved in cell growth arrest, senescence and tumour supression.
Synonyms	SNF2B, SNF2L4, SNF2-beta, BRG-1 protein, Brahma protein homolog 1
Reference Data
Protein Families	Druggable Genome, Transcription Factors