Canine Dihydrotestosterone,DHT ELISA Kit
Référence CSB-E07881c-24T
Conditionnement : 24T
Marque : Cusabio
| Size | 96T,5×96T,10×96T |
Product Details
Target Name
Dihydrotestosterone,DHT
Abbreviation
DHT
Species
Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types
serum, plasma, tissue homogenates
Detection Range
40 pg/mL-8000 pg/mL
Sensitivity
20 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Competitive
Precision
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of canine DHT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 93-103 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:4 | Average % | 102 | ||||
| Range % | 98-104 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 82-95 | |||||
Recovery
| The recovery of canine DHT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-99 | ||||
| EDTA plasma (n=4) | 99 | 98-103 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||
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ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This Dog DHT ELISA Kit was designed for the quantitative measurement of Dog DHT protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 40 pg/mL-8000 pg/mL and the sensitivity is 20 pg/mL .
Citations
- Zhao R.et al,Cell Prolif,2017
- Xing-Jie Wang.et al,THE PROSTATE,2017
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