Canine Inhibin B ELISA Kit
Référence MBS029204-96
Conditionnement : 96-Strip-Wells
Marque : MyBiosource
Inhibin B (INHB) ELISA Kit for Canine
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS029204Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated INHB concentrations in Canine serum, plasma and other body fluids. Using Purified Canine INHB antibody to coat Microelisa Stripplate… More details
Product Overview
Online Datasheet in PDFProduct Name
Inhibin B (INHB), ELISA Kit
Full Product Name
Canine Inhibin B ELISA Kit
Product Gene Name
INHB elisa kit
Species Reactivity
Canine
Assay Type
Sandwich
Samples
Serum, Plasma, Tissue Homogenate, Feces and Urine
Detection Range
15.6 pg/ml - 500 pg/ml.
Sensitivity
2.0 pg/ml.
Specificity
No significant cross-reactivity or interference between Canine INHB and analogues was observed.
Preparation and Storage
Store all reagents at 2-8 degree C
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Molecular Weight
45,122 Da
OMIM
147390
Intended Uses
This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated INHB concentrations in Canine serum, plasma and other body fluids. Using Purified Canine INHB antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add INHB and INHB antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of INHB in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Other Notes
Small volumes of INHB elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for INHB purchase
MBS029204 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Inhibin B (INHB) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing INHB. The ELISA analytical biochemical technique of the MBS029204 kit is based on INHB antibody-INHB antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect INHB antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, INHB. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Additional Details
NCBI/Uniprot data below describe general gene information for INHB. It may not necessarily be applicable to this product.
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
inhibin beta B chain preproprotein
NCBI Official Synonym Full Names
inhibin, beta B
NCBI Official Symbol
INHBB
UniProt Protein Name
Inhibin beta B chain
UniProt Synonym Protein Names
Activin beta-B chain
UniProt Gene Name
INHBB
UniProt Entry Name
INHBB_HUMAN
NCBI Protein Information
inhibin beta B chain; Inhibin, beta-2; activin beta-B chain; activin AB beta polypeptide
NCBI Summary for INHB
The inhibin beta B subunit joins the alpha subunit to form a pituitary FSH secretion inhibitor. Inhibin has been shown to regulate gonadal stromal cell proliferation negatively and to have tumour-suppressor activity. In addition, serum levels of inhibin have been shown to reflect the size of granulosa-cell tumors and can therefore be used as a marker for primary as well as recurrent disease. Because expression in gonadal and various extragonadal tissues may vary severalfold in a tissue-specific fashion, it is proposed that inhibin may be both a growth/differentiation factor and a hormone. Furthermore, the beta B subunit forms a homodimer, activin B, and also joins with the beta A subunit to form a heterodimer, activin AB, both of which stimulate FSH secretion. [provided by RefSeq, Jul 2008]
Show more UniProt Comments for INHB
INHBB: Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. Belongs to the TGF-beta family.
Protein type: Secreted; Cell development/differentiation; Cytokine; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 2cen-q13
Cellular Component: extracellular space; perinuclear region of cytoplasm; extracellular region
Molecular Function: protein binding; protein homodimerization activity; growth factor activity; hormone activity; cytokine activity; transforming growth factor beta receptor binding; host cell surface receptor binding
Biological Process: fat cell differentiation; activin receptor signaling pathway; defense response; negative regulation of hepatocyte growth factor biosynthetic process; cellular response to starvation; negative regulation of insulin secretion; negative regulation of follicle-stimulating hormone secretion; regulation of apoptosis; ovarian follicle development; cellular response to insulin stimulus; response to mechanical stimulus; regulation of MAPKKK cascade; positive regulation of follicle-stimulating hormone secretion; cell differentiation; cell development; oocyte development; growth
Show more Protein type: Secreted; Cell development/differentiation; Cytokine; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 2cen-q13
Cellular Component: extracellular space; perinuclear region of cytoplasm; extracellular region
Molecular Function: protein binding; protein homodimerization activity; growth factor activity; hormone activity; cytokine activity; transforming growth factor beta receptor binding; host cell surface receptor binding
Biological Process: fat cell differentiation; activin receptor signaling pathway; defense response; negative regulation of hepatocyte growth factor biosynthetic process; cellular response to starvation; negative regulation of insulin secretion; negative regulation of follicle-stimulating hormone secretion; regulation of apoptosis; ovarian follicle development; cellular response to insulin stimulus; response to mechanical stimulus; regulation of MAPKKK cascade; positive regulation of follicle-stimulating hormone secretion; cell differentiation; cell development; oocyte development; growth
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with INHB elisa kit
| Reference Product | PubMed Publications |
|---|---|
| INHBA elisa kit | >22 publications with INHB and INHBA |
| INHA elisa kit | >16 publications with INHB and INHA |
| FST elisa kit | >8 publications with INHB and FST |
| FSHB elisa kit | >2 publications with INHB and FSHB |
| INHBC elisa kit | >2 publications with INHB and INHBC |
| ACVR1B elisa kit | >2 publications with INHB and ACVR1B |
| INHBE elisa kit | >1 publications with INHB and INHBE |
| ACVR2A elisa kit | >1 publications with INHB and ACVR2A |
Pathways associated with INHB elisa kit
| Products by Pathway | Pathway Diagram |
|---|---|
| AGE/RAGE Pathway antibodies | AGE/RAGE Pathway Diagram |
| Antagonism Of Activin By Follistatin Pathway antibodies | Antagonism Of Activin By Follistatin Pathway Diagram |
| Cytokine-cytokine Receptor Interaction Pathway antibodies | Cytokine-cytokine Receptor Interaction Pathway Diagram |
| Cytokine-cytokine Receptor Interaction Pathway antibodies | Cytokine-cytokine Receptor Interaction Pathway Diagram |
| Glycoprotein Hormones Pathway antibodies | Glycoprotein Hormones Pathway Diagram |
| Metabolism Of Proteins Pathway antibodies | Metabolism Of Proteins Pathway Diagram |
| Peptide Hormone Biosynthesis Pathway antibodies | Peptide Hormone Biosynthesis Pathway Diagram |
| Peptide Hormone Metabolism Pathway antibodies | Peptide Hormone Metabolism Pathway Diagram |
| Signal Transduction Pathway antibodies | Signal Transduction Pathway Diagram |
| Signaling By Activin Pathway antibodies | Signaling By Activin Pathway Diagram |
Diseases associated with INHB elisa kit
| Disease Name | Pubmed Publications |
|---|---|
| Neoplasms Antibodies | >28 publications with INHB and Neoplasms |
| Ovarian Diseases Antibodies | >8 publications with INHB and Ovarian Diseases |
| Adenocarcinoma Antibodies | >7 publications with INHB and Adenocarcinoma |
| Infertility, Male Antibodies | >7 publications with INHB and Infertility, Male |
| Ovarian Neoplasms Antibodies | >6 publications with INHB and Ovarian Neoplasms |
| Prostatic Neoplasms Antibodies | >4 publications with INHB and Prostatic Neoplasms |
| Weight Loss Antibodies | >3 publications with INHB and Weight Loss |
| Disease Models, Animal Antibodies | >3 publications with INHB and Disease Models, Animal |
| Breast Neoplasms Antibodies | >3 publications with INHB and Breast Neoplasms |
| Neoplasm Metastasis Antibodies | >3 publications with INHB and Neoplasm Metastasis |
Organs/Tissues associated with INHB elisa kit
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Testis Antibodies | >21 publications with INHB and Testis |
| Brain Antibodies | >10 publications with INHB and Brain |
| Uterus Antibodies | >7 publications with INHB and Uterus |
| Liver Antibodies | >6 publications with INHB and Liver |
| Pancreas Antibodies | >5 publications with INHB and Pancreas |
| Prostate Antibodies | >4 publications with INHB and Prostate |
| Kidney Antibodies | >4 publications with INHB and Kidney |
| Eye Antibodies | >3 publications with INHB and Eye |
| Nerve Antibodies | >2 publications with INHB and Nerve |
| Lung Antibodies | >2 publications with INHB and Lung |
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