CD40L / CD154 antibody [5C8] (APC)
Cat. No. GTX01484-07
Cat. No. GTX01484-07
![Anti-CD40L / CD154 antibody [5C8] (APC) used in Flow cytometry (FCM). GTX01484-07](/proxy_img/aHR0cDovL3d3dy5nZW5ldGV4LmNvbS91cGxvYWQvd2Vic2l0ZS9wcm91Y3RfaW1nL21pZGRsZS9HVFgwMTQ4NC0wNy9HVFgwMTQ4NC0wN18yMDIwMDQyOF9GQUNTMjRfd18yMzA1MzEyMV84OTUud2VicA==.webp)
GTX01484-07 FCM Image
FACS analysis of human PBMCs (with PMA and Ionomycin stimulation for 5 hrs) using GTX01484-07 CD40L / CD154 antibody [5C8] (APC).
Solid lone : primary antibody
Dashed line : isotype control
antibody amount : 0.25 μg (5 μl)
Summary
The 5C8 antibody reacts with human CD154 which is also known as CD40L (CD40 ligand). CD154 is a 39kDa member of the TNF-α superfamily and is predominantly expressed on activated T cells but is also expressed on NK cells, mast cells, basophils, and eosinophils. CD154 binds to CD40, a molecule that is expressed on antigen presenting cells including B cells, monocytes/macrophages, and dendritic cells. The interaction of CD154 and CD40 regulates the activation of antigen presenting cells and is critical for B cell costimulation. The 5C8 antibody has been reported to block CD40L-CD40 interactions.