CF®488A Goat Anti-Mouse IgG2b (γ2b), 2mg/mL
Référence 20266-1
Conditionnement : 50uL
Marque : Biotium
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Téléphone : +1 850 650 7790
Goat Anti-Mouse IgG2b (γ2b)
Goat anti-mouse IgG2b isotype-specific secondary antibody labeled with our superior CF® Dyes and biotin.
Product Attributes
| Antibody type | Secondary |
|---|---|
| Clonality | Polyclonal |
| Host species | Goat |
| Antibody reactivity (target) | Mouse IgG2b (γ2b) |
| Species reactivity | Mouse |
| Cross adsorption | Bovine, Human, Rabbit |
| Concentration | 2 mg/mL |
| Antibody/conjugate formulation | Liquid: PBS/50% glycerol/2 mg/mL BSA/0.05% azide, Lyophilized: PBS/15 mg/mL BSA/20 mg/mL trehalose after reconstitution |
| Secondary/tag antibody applications | ELISA, Flow cytometry, IHC, IF (cells or tissue sections), Western blot |
Product Description
This is a goat anti-mouse IgG2b isotype-specific secondary antibody labeled with our bright and photostable CF® Dyes and biotin. The conjugates are prepared from affinity-purified antibodies that react with Fc portion of the heavy chain of mouse IgG2b. To minimize cross reactivity, the antibodies are cross-adsorbed against other mouse IgG subclasses (IgG1, IgG2a, IgG3), and human, bovine and rabbit serum proteins.
- Cross-adsorbed for specific staining with minimal background
- Available in 13 bright and photostable CF® Dyes
- Biotin conjugate also available
- Suitable for western, immunofluorescence, and immunohistology
Note: Conjugates of blue fluorescent dyes like CF®350 and CF®405S are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Goat Anti-Mouse IgG2b
| Conjugation | Ex/Em | Size | Catalog No. |
|---|---|---|---|
| CF®350 | 347/448 nm | 50 uL (100 ug) | 20265-1 |
| 0.25 mL (500 ug) | 20265 | ||
| CF®405S | 404/431 nm | 50 uL (100 ug) | 20382-50uL |
| 0.25 mL (500 ug) | 20382-250uL | ||
| CF®488A | 490/515 nm | 50 uL (100 ug) | 20266-1 |
| 0.25 mL (500 ug) | 20266 | ||
| CF®543 | 541/560 nm | 50 uL (100 ug) | 20327-1 |
| 0.25 mL (500 ug) | 20327 | ||
| CF®555 | 555/565 nm | 50 uL (100 ug) | 20267-1 |
| 0.25 mL (500 ug) | 20267 | ||
| CF®568 | 562/583 nm | 50 uL (100 ug) | 20268-1 |
| 0.25 mL (500 ug) | 20268 | ||
| CF®594 | 593/614 nm | 50 uL (100 ug) | 20269-1 |
| 0.25 mL (500 ug) | 20269 | ||
| CF®633 | 630/650 nm | 50 uL (100 ug) | 20270-1 |
| 0.25 mL (500 ug) | 20270 | ||
| CF®640R | 642/662 nm | 50 uL (100 ug) | 20271-1 |
| 0.25 mL (500 ug) | 20271 | ||
| CF®647 | 650/665 nm | 50 uL (100 ug) | 20272-1 |
| 0.25 mL (500 ug) | 20272 | ||
| CF®680 | 681/698 nm | 50 uL (100 ug) | 20273-1 |
| 0.25 mL (500 ug) | 20273 | ||
| CF®750 | 755/777 nm | 50 uL (100 ug) | 20430-50uL |
| 250 uL (100 ug) | 20430-250uL | ||
| CF®770 | 770/797 nm | 50 uL (100 ug) | 20274-1 |
| 0.25 mL (500 ug) | 20274 | ||
| Biotin | N/A | 50 uL (100 ug) | 20473-50uL |
| 0.25 mL (500 ug) (500 ug) | 20473-250uL |
View our full selection of bright and specific Secondary Antibodies, or search our catalog using our Antibody Finder. Alternatively, you can view our secondary antibody product listings with catalog numbers.
CF® Dyes offer exceptional brightness and photostability. For more information see our CF® Dye technology page.
Storage and Handling
Liquid format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Liquid format antibodies contain 50% glycerol and will not freeze at -20°C.
Lyophilized format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Reconstitute antibodies in water using the indicated volumes below:
CF® Dye and biotin conjugates: add 0.5 mL dH2O
HRP or DNP conjugates: add 1 mL dH2O
Add the indicated volume of water directly to the vial containing the lyophilized antibody and mix gently to dissolve. Store reconstituted antibody at -20°C and protect from light. Aliquot to avoid repeated freeze/thaw cycles. Alternatively, an equal volume of glycerol can be mixed with the reconstituted antibody so that it will remain liquid at -20°C.
Optional: A preservative such as 0.05% sodium azide (final concentration) can be added to CF® Dye and biotin conjugates. Do not add sodium azide to HRP conjugates.
Note: Storage of the antibody for more than a day at final working dilution is not recommended.
CF is a registered trademark of Biotium, Inc.
References
Download a list of CF® dye references.