CF®640R Goat Anti-Mouse IgG (H+L), 2 mg/mL

Référence 20197-1

Conditionnement : 50uL

Marque : Biotium

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Téléphone : +1 850 650 7790

Goat Anti-Mouse IgG (H+L)

Goat anti-mouse IgG (H+L) secondary antibody labeled with our superior CF® dyes and other labels.

Product Attributes

Antibody type

Secondary

Clonality

Polyclonal

Host species

Goat

Antibody reactivity (target)

Mouse IgG

Species reactivity

Mouse

Cross adsorption

Not cross-adsorbed

Concentration

2 mg/mL, 1 mg/mL (HRP, AP conjugates), 0.5 mg/mL (R-PE, APC conjugates)

Antibody/conjugate formulation

Liquid: PBS/50% glycerol/2 mg/mL BSA/0.05% azide, Lyophilized: PBS/15 mg/mL BSA/20 mg/mL trehalose after reconstitution, R-PE conjugates: PBS/2 mg/mL BSA/0.05% azide, HRP conjugates: PBS/50% glycerol/15 mg/mL BSA, HRP conjugates (lyophilized): PBS/10 mg/mL trehalose after reconstitution

Secondary/tag antibody applications

ELISA, Flow cytometry, IHC, IF (cells or tissue sections), Western blot

Product Description

This is a goat anti-mouse IgG (H L) secondary antibody labeled with our superior CF® dyes.

  • Available in 18 bright and photostable CF® dyes
  • Alkaline phosphatase, APC, HRP, R-PE and biotin conjugates also available
  • Suitable for western, immunofluorescence, and immunohistology in FFPE tissues

Note: Conjugates of blue fluorescent dyes like CF®350, CF®405S, and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Goat Anti-Mouse IgG (H+L)

Conjugation Ex/Em Size Catalog No.
CF®350 347/448 nm 50 uL (100 ug) 20140-1
0.5 mL (1 mg) 20140
1 mg (lyophilized) 20140-1mg
CF®405S 404/431 nm 50 uL (100 ug) 20080-1
0.5 mL (1 mg) 20080
1 mg (lyophilized) 20080-1mg
CF®405M 408/452 nm 50 uL (100 ug) 20180-1
0.5 mL (1 mg) 20180
1 mg (lyophilized) 20180-1mg
CF®405L 395/545 nm 50 uL (100 ug) 20408-50uL
0.5 mL (1 mg) 20408-500uL
1 mg (lyophilized) 20408-1mg
CF®488A 490/515 nm 50 uL (100 ug) 20010-1
0.5 mL (1 mg) 20010
1 mg (lyophilized) 20010-1mg
CF®514 516/548 nm 50 uL (100 ug) 20386-50uL
0.5 mL (1 mg) 20386-500uL
1 mg (lyophilized) 20386-1mg
CF®532 527/558 nm 50 uL (100 ug) 20365-50uL
0.5 mL (1 mg) 20365-500uL
1 mg (lyophilized) 20365-1mg
CF®543 541/560 nm 50 uL (100 ug) 20306-1
0.5 mL (1 mg) 20306
1 mg (lyophilized) 20306-1mg
CF®555 555/565 nm 50 uL (100 ug) 20030-1
0.5 mL (1 mg) 20030
1 mg (lyophilized) 20030-1mg
CF®568 562/583 nm 50 uL (100 ug) 20100-1
0.5 mL (1 mg) 20100
1 mg (lyophilized) 20100-1mg
CF®594 593/614 nm 50 uL (100 ug) 20110-1
0.5 mL (1 mg) 20110
1 mg (lyophilized) 20110-1mg
CF®633 630/650 nm 50 uL (100 ug) 20120-1
0.5 mL (1 mg) 20120
1 mg (lyophilized) 20120-1mg
CF®640R 642/662 nm 50 uL (100 ug) 20197-1
0.5 mL (1 mg) 20197
1 mg (lyophilized) 20197-1mg
CF®647 650/665 nm 50 uL (100 ug) 20040-1
0.5 mL (1 mg) 20040
1 mg (lyophilized) 20040-1mg
CF®660R 663/682 nm 50 uL (100 ug) 20054-1
0.5 mL (1 mg) 20054
1 mg (lyophilized) 20054-1mg
CF®660C 667/685 nm 50 uL (100 ug) 20050-1
0.5 mL (1 mg) 20050
1 mg (lyophilized) 20050-1mg
CF®750 755/777 nm 50 uL (100 ug) 20070-1
0.5 mL (1 mg) 20070
1 mg (lyophilized) 20070-1mg
CF®790 784/806 nm 50 uL (100 ug) 20378-50uL
R-PE 496, 546, 565/578 nm 200 uL (100 ug) 20352-200uL
1 mL (500 ug) 20352-1mL
APC 650/660 nm 100 uL (50 ug) 20411-100uL
0.5 mL (250 ug) 20411-0.5mL
Biotin N/A 50 uL (100 ug) 20183-1
0.5 mL (1 mg) 20183
1 mg (lyophilized) 20183-1mg
HRP N/A 100 uL (100 ug) 20400-100uL
1 mL (1 mg) 20400-1mL
1 mg (lyophilized) 20400-1mg
AP N/A 100 uL (100 ug) 20464-100uL
1 mL (1 mg) 20464-1mL

View our full selection of Secondary Antibodies, or search our catalog using our Antibody Finder. Alternatively, you can view our secondary antibody product listings with catalog numbers.

CF® Dyes offer exceptional brightness and photostability. For more information see our CF® Dye technology page.

Storage and Handling

Liquid format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Liquid format antibodies contain 50% glycerol and will not freeze at -20°C.

Lyophilized format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Reconstitute antibodies in water using the indicated volumes below:

CF® Dye and biotin conjugates: add 0.5 mL dH2O

HRP or DNP conjugates: add 1 mL dH2O

Add the indicated volume of water directly to the vial containing the lyophilized antibody and mix gently to dissolve. Store reconstituted antibody at -20°C and protect from light. Aliquot to avoid repeated freeze/thaw cycles. Alternatively, an equal volume of glycerol can be mixed with the reconstituted antibody so that it will remain liquid at -20°C.

Optional: A preservative such as 0.05% sodium azide (final concentration) can be added to CF® Dye and biotin conjugates. Do not add sodium azide to HRP conjugates.

Note: Storage of the antibody for more than a day at final working dilution is not recommended.

CF is a registered trademark of Biotium, Inc.

References

Download a list of CF® dye references.

 

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