Creatine Transporter (CRT) BioAssay™ ELISA Kit (Rat)
Référence 589164-96Tests
Conditionnement : 96Tests
Marque : US Biological
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Téléphone : +1 850 650 7790
Shipping Temp
Blue IceStorage Temp
4°C/-20°CThe BioAssay™kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of creatine transporter in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Detection Range:
24.69-2,000pg/ml
Sensitivity:
8.55pg/ml
Specificity:
This assay has high sensitivity and excellent specificity for detection of Creatine Transporter (CRT). No significant cross-reactivity or interference between Creatine Transporter (CRT) and analogues was observed.
Precision:
Intra-Assay %CV: <10%
Inter-Assay %CV: <12%
Inter-Assay %CV: <12%
Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to creatine transporter has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled creatine transporter and unlabeled creatine transporter (Standards or samples) with the pre-coated antibody specific to creatine transporter. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of creatine transporter in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of creatine transporter in the sample.
Kit Components:
Pre-coated, ready to use 96-well strip plate
Standard, 2x vials
Detection Reagent A, 1x120ul
Detection Reagent B, 1x120ul
TMB Substrate, 1x9ml
Wash Buffer (30X): 1x20ml
Standard Diluent, 1x20ml
Assay Diluent A, 1x12ml
Assay Diluent B, 1x12ml
Stop Solution, 1x6ml
Standard, 2x vials
Detection Reagent A, 1x120ul
Detection Reagent B, 1x120ul
TMB Substrate, 1x9ml
Wash Buffer (30X): 1x20ml
Standard Diluent, 1x20ml
Assay Diluent A, 1x12ml
Assay Diluent B, 1x12ml
Stop Solution, 1x6ml
Storage and Stability:
Store Microtiter Strips, Standard, Detection Reagents and Positive Control at -20°C. After reconstitution of Positive Control, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Assay Procedure Summary:
1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to each well. Incubate 1 hour at 37°C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.
6. Aspirate and wash 5 times.
7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37°C.
8. Add 50ul Stop Solution. Read at 450nm immediately.
2. Add 100ul standard or sample to each well. Incubate 1 hour at 37°C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.
6. Aspirate and wash 5 times.
7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37°C.
8. Add 50ul Stop Solution. Read at 450nm immediately.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.