Dengue virus NS1 protein antibody
Référence GTX103346-100ul
Conditionnement : 100ul
Marque : Genetex
Contactez votre distributeur local :
Téléphone : +1 850 650 7790
3
Host | Rabbit |
|---|---|
Clonality | Polyclonal |
Isotype | IgG |
Application | WB, ICC/IF, ELISA |
Reactivity | Dengue virus 2 |
| Package | 100 μl, 25 μl |
Datasheet File View product citations for antibody GTX103346 on CiteAb
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application | Recommended Dilution |
|---|---|
| WB | 1:500-1:3000 |
| ICC/IF | 1:1000-1:2000 |
| ELISA | Assay dependent |
Not tested in other applications.
Calculated MW
40 kDa. ( Note )
Positive Control
Dengue virus 2-infected BHK-21
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Dengue virus 2
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of Dengue virus NS1 protein (Dengue virus 2 (strain 16681 PDK 53)). The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1240703
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Dengue Virus Non-structural protein 1 , Dengue Virus NS1 , DENV Non-structural protein 1 , DENV NS1
DATA IMAGES
| | GTX103346 WB ImageNon-infected (–) and infected (+) Huh-7 whole cell extracts (15 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Dengue virus NS1 protein antibody (GTX103346) diluted at 1:2000. |
| | GTX103346 ICC/IF ImageGTX103346 IF Image Immunofluorescence analysis of Denque virus 2-infected BHK-21, using NS1 (Dengue virus) antibody (GTX103346) at 1:2000 dilution. |
| | GTX103346 WB ImageNon-infected (–) and infected (+) BHK-21 whole cell extracts (15 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Dengue virus NS1 protein antibody (GTX103346) diluted at 1:1000. |
Application Reference
Udawatte DJ et al. Front Cell Infect Microbiol 2022; 12 : 926036 Dengue virus downregulates TNFR1- and TLR3-stimulated NF-κB activation by targeting RIPK1.
Application : WB
Xie X et al. Cell Host Microbe 2019; 26 (5) : 606-622.e8 Dengue NS2A Protein Orchestrates Virus Assembly.
Application : WB
Lai JH et al. EMBO Rep 2018; (Epub) Infection with the dengue RNA virus activates TLR9 signaling in human dendritic cells.
Application : WB
Callaway JB et al. PLoS One 2015; 10 (8) : e0136708 Source and Purity of Dengue-Viral Preparations Impact Requirement for Enhancing Antibody to Induce Elevated IL-1β Secretion: A Primary Human Monocyte Model.
Tham HW et al. J Infect Dev Ctries 2015; 9 (12) : 1338-49 Protein-protein interactions between A. aegypti midgut and dengue virus 2: two-hybrid screens using the midgut cDNA library.
Submit a Reference Application : ICC/IF
REVIEW
Dengue virus NS1 protein antibody Cat. No. GTX103346
| Rating | | ( Average 5 based on 3 users reviews) |
| Western Blot(WB) | | ( Average 5 based on 3 users reviews) |
Application Reference
Udawatte DJ et al. Front Cell Infect Microbiol 2022; 12:926036 Dengue virus downregulates TNFR1- and TLR3-stimulated NF-κB activation by targeting RIPK1.
Application : WB
Xie X et al. Cell Host Microbe 2019; 26 (5):606-622.e8 Dengue NS2A Protein Orchestrates Virus Assembly.
Application : WB
Lai JH et al. EMBO Rep 2018; (Epub) Infection with the dengue RNA virus activates TLR9 signaling in human dendritic cells.
Application : WB
Callaway JB et al. PLoS One 2015; 10 (8):e0136708 Source and Purity of Dengue-Viral Preparations Impact Requirement for Enhancing Antibody to Induce Elevated IL-1β Secretion: A Primary Human Monocyte Model.
Tham HW et al. J Infect Dev Ctries 2015; 9 (12):1338-49 Protein-protein interactions between A. aegypti midgut and dengue virus 2: two-hybrid screens using the midgut cDNA library.
Application : ICC/IF
Hsu AY et al. Sci Rep 2015; 5:17990 Infectious dengue vesicles derived from CD61+ cells in acute patient plasma exhibited a diaphanous appearance.
Application : WB
Xie X et al. Virology 2014; 450-451:250-7 Generation and characterization of mouse monoclonal antibodies against NS4B protein of dengue virus.
Application : WB, ICC/IF
Zou J et al. J Virol 2014; 88 (6):3379-91 Dimerization of Flavivirus NS4B Protein.
Application : WB
Sridharan A et al. J Virol 2013; 87 (21):11648-58 Inhibition of megakaryocyte development in the bone marrow underlies dengue virus-induced thrombocytopenia in humanized mice.
Application : WB
Lin SW et al. J Infect 2012; 64 (3):325-34 Dengue virus nonstructural protein NS1 binds to prothrombin/thrombin and inhibits prothrombin activation.
Application : WB, ELISA
| SDS | |
|---|---|
| PBS.pdf | |
| Glycerol.pdf | |
| Proclin.pdf | |
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