DIO1 ELISA Kit (Chicken)
Référence OKEH07820
Conditionnement : 96T
Marque : Aviva Systems Biology
DIO1 ELISA Kit (Chicken) (OKEH07820)
| Datasheets/Manuals | Printable datasheet for DIO1 ELISA Kit (Chicken) (OKEH07820) |
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| Predicted Species Reactivity | Chicken|Gallus gallus | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology DIO1 ELISA Kit (Chicken) (OKEH07820) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DIO1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DIO1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DIO1 captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 92% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: <=9.3% (n = 20) Mean Inter-assay CV%: <=9.4% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Component |
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| Additional Information | Function: Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine) and of T3 into T2 (3,3'-diiodothyronine). | ||||||||||||||||||||||
| :: | Subcellular Location: Endoplasmic reticulum membrane Single-pass membrane protein | ||||||||||||||||||||||
| :: | Unigene: Gga.553 String: 9031.ENSGALP00000017451 | ||||||||||||||||||||||
| :: | KEGG: gga:395940 Pfam: PF00837 | ||||||||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sensitivity | 0.056ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Chicken Type I iodothyronine deiodinase | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | DIO1 |
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| Gene Full Name | deiodinase, iodothyronine type I |
| Alias Symbols | 5DI, DIOI, iodothyronine deiodinase 1, type 1 DI, type I iodothyronine deiodinase, type-I 5'-deiodinase. |
| NCBI Gene Id | 395940 |
| Protein Name | Type I iodothyronine deiodinase |
| Description of Target | Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine) and of T3 into T2 (3,3'-diiodothyronine). |
| Uniprot ID | O42411 |
| Protein Accession # | NP_001091083.1 |
| Nucleotide Accession # | NM_001097614.1 |